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pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
UseTags6xHIS on N terminus of Csm2ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c4X
Plasmid#75288PurposeExpresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSKDuet01
Plasmid#12172DepositorInsertNpuDnaE
UseTagsHis-GB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPet-His
Plasmid#14031DepositorInsertYPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSKBAD2
Plasmid#15335DepositorInsertDnaE C-intein
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJBL7010
Plasmid#136942PurposeConstitutive sfGFP expression (high), bacterial promoterDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64T (DM#111)
Plasmid#15030DepositorInsertbeta-globin 5' and 3' UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
Venus-pBAD
Plasmid#54859PurposeLocalization: Protein Expression Vector , Excitation: 515, Emission: 528DepositorTypeEmpty backboneUseTags6xHis and VenusExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2H12Matry
Plasmid#221143PurposecdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)DepositorInsertcdGreen-Matry
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxGFP
Plasmid#103984PurposePlasmid encoding moxGFPDepositorInsertmoxGFP
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-pBAD
Plasmid#54666DepositorTypeEmpty backboneUseTags6xHis and mCeruleanExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYX-C-mScarlet-Zeo-TSC22D2
Plasmid#222670Purposeendogenously tag TSC22D2 with C-terminal mScarletDepositorInsertTSC22D2 (TSC22D2 Human)
UseTagsmScarletExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAK501
Plasmid#48107PurposelacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistanceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pTarget
Plasmid#214052PurposeExpresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.DepositorInsertTarget sequence
UseTarget rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNon-target
Plasmid#214053PurposeExpresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.DepositorInsertNon-target sequence
UseNon-target rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
UseTagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQH
Plasmid#48096PurposeCumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAG and HAExpressionBacterialMutationPromoterPQ5Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
UseTagsExpressionBacterialMutationPromoterPEM7 and unknownAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorInsertHIS-HA-CMYC-p53 (TP53 Human)
UseTagsHIS-HA-CMYCExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneUseTagsHis6 and Twin-Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP1H-mCf
Plasmid#229984PurposeLIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneUseTagsStrep-tag IIExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP3H-mCf
Plasmid#229987PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
UseTags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFGET19_Ulp1
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
UseTags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
tFucci(CA)5
Plasmid#153521PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.DepositorInserttFucci(CA)5.
UseTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
UseTagsExpressionBacterialMutationPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTn Donor
Plasmid#236211PurposeArabinose-inducible Tn5 InducTn-seq plasmidDepositorInsertNone
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGRG36
Plasmid#16666DepositorInsertmTn7::MCS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCre
Plasmid#236212PurposeArabinose-inducible Cre recombinaseDepositorInsertCre recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only
