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32,126 results

Showing: 1641 - 1680 of 32126 results

  4. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  8. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  9. pAP114

    Plasmid
    #120799
    Purpose
    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Insert
    Pxyl::mCherryOpt
    Expression
    Bacterial
    Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…
    Promoter
    Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120799-plasmid-map-sequence-id-235314

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  14. pBAD-mLemon

    Plasmid
    #191256
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein mLemon
    Depositor
    Robert Campbell
    Insert
    mLemon
    Tags
    His tag
    Expression
    Bacterial
    Available Since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    191256-plasmid-map-sequence-id-378486

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  17. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

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  18. Cys-60nmSAH-SPYcatcher

    Plasmid
    #214840
    Purpose
    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    Cys-60nmSAH-SPYcatcher
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214840-plasmid-map-sequence-id-425312

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  20. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  23. Pet28a_Human_H3.3

    Plasmid
    #42632
    Depositor
    Joe Landry
    Insert
    Human H3.3 (H3-3A Human)
    Tags
    Untagged
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42632-plasmid-map-sequence-id-57747

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  29. pVHD

    Plasmid
    #61303
    Purpose
    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag
    Depositor
    Julia Vorholt
    Article
    Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103
    Type
    Empty backbone
    Tags
    HA
    Expression
    Bacterial
    Promoter
    PV10
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61303-plasmid-map-sequence-id-120729

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  32. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Promoter
    PhaR promoter
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

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  34. pQE60 MGH

    Plasmid
    #204244
    Purpose
    Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MGH
    Tags
    6X His and GFP
    Expression
    Bacterial
    Mutation
    fusion of MDH and GFP with His tag
    Promoter
    Lac
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204244-plasmid-map-sequence-id-411051

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Showing: 1641 - 1680 of 32126 results