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31,059 results

Showing: 1961 - 2000 of 31059 results

  1. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  2. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

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  3. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid
    #176573
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176573-plasmid-map-sequence-id-348271

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  7. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

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  13. SARS_pNIC-CTB10H

    Plasmid
    #153056
    Purpose
    Expression construct for producing Avi tagged Seryl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    SARS (SARS1 Human)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153056-plasmid-map-sequence-id-304476

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  16. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

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  18. pQE81XN-mGold

    Plasmid
    #218959
    Purpose
    Expresses Hisx6-Tagged mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    mGold
    Tags
    6X-His
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218959-plasmid-map-sequence-id-445675

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  20. pRSF-MjCouRS

    Plasmid
    #229066
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024
    Insert
    Methanococcus jannaschii tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229066-plasmid-map-sequence-id-459819

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  27. pCAG-GFP-CD63

    Plasmid
    #194989
    Purpose
    overexpress CD63
    Depositor
    Angelique Bordey
    Insert
    GFP-CD63
    Expression
    Bacterial
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194989-plasmid-map-sequence-id-387085

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  31. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans trpC promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

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  34. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  39. pDonor_RB-TnV2

    Plasmid
    #213907
    Purpose
    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    James Collins
    Article
    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.
    Insert
    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    Expression
    Bacterial
    Available Since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213907-plasmid-map-sequence-id-422622

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Showing: 1961 - 2000 of 31059 results