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32,120 results

Showing: 1961 - 2000 of 32120 results

  5. p1A

    Plasmid
    #162692
    Purpose
    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162692-plasmid-map-sequence-id-324151

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  9. pGLO-Yellow-AZA

    Plasmid
    #220783
    Purpose
    Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    yellow chromoprotein
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    220783-plasmid-map-sequence-id-444833

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  11. p406TEF1

    Plasmid
    #15976
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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  16. pRibo-Tt

    Plasmid
    #154124
    Purpose
    Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells
    Depositor
    Alexander Mankin
    Article
    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:
    Inserts
    engineered bacterial rRNAs operon under control of pL promotor
    bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Ptac and pL
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154124-plasmid-map-sequence-id-308059

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  18. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

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  20. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  22. pET-FleBt

    Plasmid
    #149302
    Purpose
    "Enhances the yields of soluble target proteins in E. coli expression system", "Enable quick and efficient protein purification with high purity and stability"
    Depositor
    Michael Kolbe
    Article
    Wang et al PLoS Pathog. 2021 Nov 17;17(11):e1010052
    Type
    Empty backbone
    Tags
    FleBt and HRV 3C protease recognition site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    149302-plasmid-map-sequence-id-311624

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  24. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

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  27. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60717-plasmid-map-sequence-id-97574

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  30. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  31. pCV019-catP

    Plasmid
    #232825
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol
    Depositor
    Athanasios Typas
    Insert
    catP
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232825-plasmid-map-sequence-id-462956

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  32. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

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Showing: 1961 - 2000 of 32120 results