30,003 results

1. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

   Plasmid

   #228636

   Purpose

   Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Enterovirus A71 3C protease isoform B
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

   Plasmid

   #215759

   Purpose

   HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

   Insert

   CD247-truncCD19CAR-GSG (CD19, CD247 Human)
   

   Use

   Tags

   IgG1 CH3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

   Plasmid

   #204810

   Purpose

   Bacterial expresison of Enterovirus A71 2A protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   A71 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pHH0103_GRB2_SH3

   Plasmid

   #110015

   Purpose

   Protein expression and purification of GRB2_SH3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GRB2_SH3 (GRB2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMAL-AGR2

   Plasmid

   #18097

   Depositor

   Ronny Drapkin

   Insert

   AGR2 (AGR2 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBAD-LSSmCherry1

   Plasmid

   #89986

   Purpose

   Long stokes-shift variant of red fluorescent protein mCherry

   Depositor

   Robert Campbell

   Article

   Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

   Insert

   LSSmCherry1
   

   Use

   Tags

   6xHis-T7-Xpress

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 E protein (E SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 M Protein (M SARS-COV-2, Synthetic, Human)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMycoFos

    Plasmid

    #84577

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

    Depositor

    Nicholas Coleman

    Article

    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX6P1-HA-MEK1

    Plasmid

    #53159

    Purpose

    Expresses GST-HA tagged human MEK1 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT85_SpdCas9_pmcDA1

    Plasmid

    #204614

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTOX2

    Plasmid

    #127447

    Purpose

    Encodes MqsR toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    mqsR toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PARG-c002

    Plasmid

    #173107

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARG (PARG Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S448-T976

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV/SB10

    Plasmid

    #24551

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Use

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-c2X_MBP-CLmN-H6

    Plasmid

    #234487

    Purpose

    Maltose binding protein N-terminally fused to His-tagged CLmN split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    N-terminal Fragment of the CLm Split Intein
    

    Use

    Tags

    Hexahistidine Tag and Maltose Binding Protein (MB…

    Expression

    Bacterial

    Mutation

    T69K, F75H, M118N within the N-terminal fragment …

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAD43-daf-16 RNAi

    Plasmid

    #34833

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pET/ELP-I-CAT

    Plasmid

    #71461

    Purpose

    ELP-intein purification system

    Depositor

    David Wood

    Article

    Banki et al Nat Methods. 2005 Sep;2(9):659-61.

    Type

    Empty backbone

    Use

    Tags

    ELP ((VPGXG)110) and intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJL1-sfGFP

    Plasmid

    #102634

    Purpose

    In vitro expression of sfGFP from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    sfGFP
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSP64TS (DM#117)

    Plasmid

    #15034

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge