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32,115 results

Showing: 2041 - 2080 of 32115 results

  1. pACM4-ACA

    Plasmid
    #49809
    Purpose
    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Expression
    Bacterial
    Mutation
    Silent site-directed mutagenesis to remove SalI s…
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49809-plasmid-map-sequence-id-74624

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  3. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  4. pGEX-6P-Cdh1wt (570)

    Plasmid
    #39877
    Depositor
    Jonathon Pines
    Insert
    FZR1 (FZR1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pConRef-2H12

    Plasmid
    #221182
    Purpose
    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221182-plasmid-map-sequence-id-437177

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  8. SPLICSFAST-NU-MT-P2A

    Plasmid
    #214898
    Purpose
    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-NU-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214898-plasmid-map-sequence-id-423484

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  9. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  16. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85502-plasmid-map-sequence-id-169860

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  17. pT2/HB

    Plasmid
    #26557
    Depositor
    Perry Hackett
    Insert
    pT2/HB
    Expression
    Bacterial
    Available Since
    Dec. 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    26557-plasmid-map-sequence-id-352286

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  18. pUBC-SV40NLS-HoTag3-Shunzhi12nm-TSapphire

    Plasmid
    #250617
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 12nm nanoGEMs under the UBC promoter
    Depositor
    Liam Holt
    Insert
    12nm Shunzhi 60mers
    Use
    Lentiviral
    Tags
    4X(GGGGS) linker, HOTag3, SV40NLS, and TSapphire
    Expression
    Bacterial and Mammalian
    Promoter
    pUBC
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    250617-plasmid-map-sequence-id-497478

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  19. pEM407

    Plasmid
    #248739
    Purpose
    pPhlF133 driving sfGFP in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP2E5-RBS1PLP-PhlF-rrnBT1-T7Te-pPhlF133-RBS1PLP-sfGFP-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248739-plasmid-map-sequence-id-496789

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  20. pEM405

    Plasmid
    #248738
    Purpose
    pTetR56 driving sfGFP in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP2E5-RBS1PLP-TetR-rrnBT1-pTetR56-RBS1PLP-sfGFP-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248738-plasmid-map-sequence-id-496780

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  21. pEM403

    Plasmid
    #248736
    Purpose
    pLacI43 driving sfGFP in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP2E5-RBS1PLP-LacI-rrnBT1-pLacI43-RBS1PLP-sfGFP-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248736-plasmid-map-sequence-id-496776

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  29. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  30. pSUC01

    Plasmid
    #177204
    Purpose
    chromogenic reporter for gene knockout in Streptomyces
    Depositor
    Wenjun Guan
    Article
    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1
    Insert
    SaindC
    Use
    Synthetic Biology
    Tags
    NONE
    Expression
    Bacterial
    Mutation
    NONE
    Promoter
    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    177204-plasmid-map-sequence-id-382362

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  34. SpyLigase

    Plasmid
    #51722
    Purpose
    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SpyLigase
    Tags
    His6 Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51722-plasmid-map-sequence-id-251770

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  35. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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Showing: 2041 - 2080 of 32115 results