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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEXTK-Pak1 70-117

Plasmid

#12217

Depositor

Jonathan Chernoff

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Bacterial

Mutation

PBD (p21-binding domain), aa 70-117

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Publication

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET29b-10xHis-Super TEV

Plasmid

#193833

Purpose

Expresses high-efficiency TEV protease in bacterial cells

Depositor

EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

Publication

Lau et al protocols.io, doi: 10.17504/protocols.io

Insert

TEV protease

Tags

6xHis

Expression

Bacterial

Mutation

T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGRG36

Plasmid

#16666

Depositor

Nancy Craig

Publication

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::MCS

Expression

Bacterial

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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mCherry-pBAD

Plasmid

#54630

Purpose

Localization: Protein Expression Vector , Excitation: 587, Emission: 610

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and mCherry

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Publication

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Publication

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Publication

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCS2TAL3-DD

Plasmid

#37275

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

Depositor

David Grunwald

Publication

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

Flag, Fok I DD, Tal-C, and Tal-N

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Publication

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Publication

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCS2TAL3-RR

Plasmid

#37276

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

Depositor

David Grunwald

Publication

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pET His6 Sumo TEV LIC cloning vector (2S-T)

Plasmid

#29711

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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miniTurbo-His6_pET21a

Plasmid

#107178

Purpose

expresses His6-tagged miniTurbo in bacteria

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Bacterial

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Publication

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Publication

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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