33,445 results

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGEXTK-Pak1 70-117

Plasmid

#12217

Depositor

Jonathan Chernoff

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Bacterial

Mutation

PBD (p21-binding domain), aa 70-117

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET29b-10xHis-Super TEV

Plasmid

#193833

Purpose

Expresses high-efficiency TEV protease in bacterial cells

Depositor

EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

Article

Lau et al protocols.io, doi: 10.17504/protocols.io

Insert

TEV protease

Tags

6xHis

Expression

Bacterial

Mutation

T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGRG36

Plasmid

#16666

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::MCS

Expression

Bacterial

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-pBAD

Plasmid

#54630

Purpose

Localization: Protein Expression Vector , Excitation: 587, Emission: 610

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and mCherry

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD

Plasmid

#37275

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

Depositor

David Grunwald

Article

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

Flag, Fok I DD, Tal-C, and Tal-N

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR

Plasmid

#37276

Purpose

TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

Depositor

David Grunwald

Article

Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

Type

Empty backbone

Tags

C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV LIC cloning vector (2S-T)

Plasmid

#29711

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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miniTurbo-His6_pET21a

Plasmid

#107178

Purpose

expresses His6-tagged miniTurbo in bacteria

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Bacterial

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP dG

Plasmid

#31842

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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