31,059 results
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hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR62
Plasmid#125076PurposeExpression of ompR under PLtetO-1, output promoter PompF146DepositorAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
TagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA1
Plasmid#211910PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PKMYT1
Plasmid#39061PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterrrnB P1Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG1C
Plasmid#71740PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OSK
Plasmid#136552PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p006kanGFP
Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterT5 between two lac operatorsAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
V72 ELP
Plasmid#68938PurposeElastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residueDepositorInsertV72 ELP
ExpressionBacterialPromoterT7Available SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET12a-PAPI
Plasmid#19961DepositorInsertAnnexin V (ANXA5 Human)
ExpressionBacterialAvailable SinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
ExpressionBacterialPromoterT7Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HC078 (3xHA-Atg13)
Plasmid#59544PurposeExpresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
Tags3xHAExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184741Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-8
Plasmid#228990PurposeFor bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-8
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN172
Plasmid#215291PurposeExpresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.DepositorInsertsfGFP
TagsHisExpressionBacterialPromoterJ23113Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS FGF3 isoform 1
Plasmid#19230DepositorInsertFibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse IRS-2
Plasmid#11372DepositorInsertIRS-2 (Irs2 Mouse)
ExpressionBacterialAvailable SinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHM_YEAST
Plasmid#204231PurposeBacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM_YEAST (MDH1 Budding Yeast)
Tags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
19 pCOLI_A_SUMO
Plasmid#160257PurposeN-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and SUMOExpressionBacterialPromoterT7Available SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB23
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
ExpressionBacterialPromoterhydrogen peroxide-inducible promoter katGpAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBWB424
Plasmid#140638PurposeRSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.DepositorInsertCas9
ExpressionBacterialAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
COC053
Plasmid#209131PurposeEnzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.DepositorInsertEnzyme BirA (birA E. coli)
ExpressionBacterialAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
TagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-His7tag-Peredox-mCherry
Plasmid#32382DepositorInsertHis7-Peredox-mCherry
TagsHis7tagExpressionBacterialAvailable SinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGSTag
Plasmid#21877DepositorTypeEmpty backboneTagsGSTExpressionBacterialAvailable SinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23100, Bba_J23109, and PttrB185-269Available SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-T-rnVAPA-1-219
Plasmid#13395DepositorAvailable SinceFeb. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKL2310
Plasmid#187875PurposeEmpty pVS1-based INTEGRATE vector with loxP cargoDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AsnRS
Plasmid#124105PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFG-His
Plasmid#124126PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP4639
Plasmid#107771PurposeExpression of EYFPDepositorInsertpLacZ-EYFP in pME6010
TagspLacZ-EYFPExpressionBacterialPromoterpLacZAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH
Plasmid#185713PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged W403 and F753 to APromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
ExpressionBacterialPromotercptAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyExpressionBacterialPromoterpLacIAvailable SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only
