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32,115 results

Showing: 1981 - 2010 of 32115 results

  11. pGEX-6P-Cdh1wt (570)

    Plasmid
    #39877
    Depositor
    Jonathon Pines
    Insert
    FZR1 (FZR1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pConRef-2H12

    Plasmid
    #221182
    Purpose
    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221182-plasmid-map-sequence-id-437177

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  18. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  19. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

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  20. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  21. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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  24. pACM4-ACA

    Plasmid
    #49809
    Purpose
    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Expression
    Bacterial
    Mutation
    Silent site-directed mutagenesis to remove SalI s…
    Promoter
    T7
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49809-plasmid-map-sequence-id-74624

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  25. SPLICSFAST-NU-MT-P2A

    Plasmid
    #214898
    Purpose
    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-NU-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214898-plasmid-map-sequence-id-423484

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Showing: 1981 - 2010 of 32115 results