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32,771 results

Showing: 1981 - 2010 of 32771 results

  1. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαs-LgB113 (GNAS Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  2. pMal-Strep

    Plasmid
    #209345
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    209345-plasmid-map-sequence-id-419544

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  9. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

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  10. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  25. p30T crRNA55/120

    Plasmid
    #250980
    Purpose
    Expresses two Cas13a guide RNAs targeting orf55 and orf120 in phiKZ
    Depositor
    Joseph Bondy-Denomy
    Insert
    Dual CRISPR array expressing two Cas13a crRNAs to target orf55 and orf120 in phiKZ
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250980-plasmid-map-sequence-id-500788

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  26. pUCminiTn7-Mariner-flag

    Plasmid
    #250982
    Purpose
    pUC mini-Tn7 inserting Mariner transposase-flag into genome
    Depositor
    Joseph Bondy-Denomy
    Insert
    Mariner transposase with a FLAG tag
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250982-plasmid-map-sequence-id-504698

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  27. pHERD30T-TnV1acrVIA1

    Plasmid
    #250983
    Purpose
    pHERD30T vector providing the transposon donor with acrVIA1
    Depositor
    Joseph Bondy-Denomy
    Insert
    Transposon donor V1 encoding acrVIA1
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250983-plasmid-map-sequence-id-500789

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  28. pHERD20T-V3TnAcrVIA1

    Plasmid
    #250984
    Purpose
    pHERD20T vector providing the transposon donor with acrVIA1
    Depositor
    Joseph Bondy-Denomy
    Insert
    Transposon donor V3 encoding acrVIA1
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250984-plasmid-map-sequence-id-500738

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Showing: 1981 - 2010 of 32771 results