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Bacterial Expression

29,712 results

Showing: 3301 - 3350 of 29712 results

  1. pMB1-A-TelR

    Plasmid
    #204979
    Purpose
    Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
    Depositor
    Veronica Godoy-Carter
    Article
    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Added replications elements for Acinetobacter spe…
    Promoter
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    204979-plasmid-map-sequence-id-404862

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  5. pOPS0254

    Plasmid
    #115506
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115506-plasmid-map-sequence-id-224294

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  6. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

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  7. pCDB179-Z1-PETase

    Plasmid
    #218703
    Purpose
    Encodes codon optimized Z1-PETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    Z1-PETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218703-plasmid-map-sequence-id-434220

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  8. pBP-P_tetR-PTet*

    Plasmid
    #204077
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    tetR repressor and PTet* tetracycline-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204077-plasmid-map-sequence-id-413892

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  11. Pf-ClpP

    Plasmid
    #25591
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-ClpP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  16. pET28a-Ec.coaA

    Plasmid
    #50386
    Purpose
    Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
    Depositor
    Erick Strauss
    Article
    Brand et al J Biol Chem. 2005 May 27;280(21):20185-8
    Insert
    PanK
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50386-plasmid-map-sequence-id-75331

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  21. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Use
    Tags
    E6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pETDest

    Plasmid
    #124245
    Purpose
    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124245-plasmid-map-sequence-id-257540

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  30. pDIV298

    Plasmid
    #204957
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204957-plasmid-map-sequence-id-415603

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  33. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  41. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  45. pIMP1-Pthl

    Plasmid
    #90485
    Purpose
    Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052
    Depositor
    Sheng Yang
    Article
    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90485-plasmid-map-sequence-id-178582

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  47. pUCmEos3.2:cat

    Plasmid
    #78100
    Purpose
    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
    Depositor
    Peng Chen
    Article
    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1
    Insert
    mEos3.2-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  50. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109391-plasmid-map-sequence-id-210784

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Showing: 3301 - 3350 of 29712 results