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5,013 results

Showing: 1 - 30 of 5013 results

  8. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

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  9. pFastBacDual with LFn + Needle

    Plasmid
    #84868
    Purpose
    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Needle
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84868-plasmid-map-sequence-id-162901

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  19. pOCC240-MBP-Pab1-mCherry

    Plasmid
    #219980
    Purpose
    Pab1p-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    Pab1p-mCherry (PAB1 Budding Yeast)
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219980-plasmid-map-sequence-id-436810

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  20. pFL

    Plasmid
    #110739
    Purpose
    Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site
    Depositor
    Tim Richmond
    Article
    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi
    Type
    Empty backbone
    Expression
    Insect
    Promoter
    Polyhedrin and p10 promoters
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110739-plasmid-map-sequence-id-213587

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  21. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid
    #115160
    Purpose
    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.
    Depositor
    Tobias Langenhan
    Article
    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.
    Insert
    attB-TT loxP 3xP3-DsRed
    Use
    Cre/Lox
    Expression
    Insect
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    115160-plasmid-map-sequence-id-224340

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  23. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

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  26. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

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Showing: 1 - 30 of 5013 results