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5,017 results

Showing: 551 - 600 of 5017 results

  9. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Insert
    GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41125-plasmid-map-sequence-id-54698

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  10. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid
    #185447
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185447-plasmid-map-sequence-id-364982

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  15. pDyn1

    Plasmid
    #64067
    Purpose
    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    DYNC1H1 (DYNC1H1 Human)
    Use
    Cre/Lox
    Tags
    His-ZZ-TCS-SNAPf
    Expression
    Insect
    Mutation
    Codon optimized for Sf9
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    64067-plasmid-map-sequence-id-107133

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  24. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  25. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  26. pUASp-GBP-Tbro

    Plasmid
    #179995
    Purpose
    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.
    Depositor
    Graydon Gonsalvez
    Article
    Baker et al Development. 2021 Nov 15;148(22). pii: 2
    Insert
    GBP nanobody
    Tags
    Trbo
    Expression
    Insect
    Promoter
    UAS-Gal4
    Available Since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    179995-plasmid-map-sequence-id-357336

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  28. IR25a-T2A-QF-AD

    Plasmid
    #162526
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162526-plasmid-map-sequence-id-354666

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  30. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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  31. PSL1180polyUBdsRED

    Plasmid
    #49327
    Purpose
    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    dsRED
    Expression
    Insect
    Promoter
    Aedes polyubiquitin
    Available Since
    Nov. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49327-plasmid-map-sequence-id-73022

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  35. pADC10-SETXhel

    Plasmid
    #193056
    Purpose
    Baculovirus expression of human Senataxin helicase domain for protein production
    Depositor
    Andrew Deans
    Article
    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:
    Insert
    Senataxin (SETX Human)
    Tags
    Flag
    Expression
    Insect and Mammalian
    Mutation
    codon optimized for insect cell expression
    Promoter
    hybrid p10 (insect) and CMV (human) expression
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    193056-plasmid-map-sequence-id-381199

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  38. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  41. pAc5.1B-lambdaN-HA-HseIF4AI_T

    Plasmid
    #147543
    Purpose
    Insect Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Expression
    Insect
    Mutation
    3 silent mutations compared to the sequence given…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147543-plasmid-map-sequence-id-491297

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  44. pE2c

    Plasmid
    #17462
    Purpose
    modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    3xHA
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17462-plasmid-map-sequence-id-140110

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Showing: 551 - 600 of 5017 results