4,932 results

1. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFAST-BAC BAF57/SMARCE1-His

   Plasmid

   #177861

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCE1 (SMARCE1 Human)
   

   Tags

   His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD3-frame_selector_1

   Plasmid

   #127554

   Purpose

   Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sgRNA frame 1
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

   Plasmid

   #169077

   Purpose

   Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Tags

   GFP tag, GGlinker, TEV cleavage site. StrepII and…

   Expression

   Bacterial and Insect

   Promoter

   Polyhedrin promoter

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBacHTa_HCK

   Plasmid

   #224245

   Purpose

   Expression of human HCK Kinase in insect cells (SF9 cells)

   Depositor

   Andrew Mesecar

   Insert

   hck (HCK Human)
   

   Tags

   6xHis-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASp YFP Rabx6 WT

   Plasmid

   #53479

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rabx6 (RabX6 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pENTR R4 ATCC LacZ GCTT

   Plasmid

   #173668

   Purpose

   Destination vector for Golden Gate Cloning assemblies, blue-white screening

   Depositor

   Eric Marois

   Article

   Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

   Type

   Empty backbone

   Expression

   Bacterial and Insect

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 10XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111547

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.SUZ12

   Plasmid

   #125162

   Purpose

   Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein SUZ12 (SUZ12 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCasper_Pdf-LexA::VP16

    Plasmid

    #33259

    Depositor

    Michael Rosbash

    Article

    Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

    Insert

    LexA::VP16-SV40 3'UTR (lexA E. coli)
    

    Expression

    Insect

    Promoter

    Pdf

    Available Since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pOPINE-3C-YFP

    Plasmid

    #214028

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    YFP
    

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH5

    Plasmid

    #52531

    Purpose

    expresses FLP recombinase in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Expression

    Insect

    Promoter

    ubiquitin

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFD3-frame_selector_2

    Plasmid

    #127555

    Purpose

    Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1-OSF-TRIM5alpha(human)

    Plasmid

    #79034

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ptubP-QS

    Plasmid

    #24352

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QS
    

    Tags

    white gene

    Expression

    Insect

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJFRC157-20XUAS-IVS-B3::PEST

    Plasmid

    #32136

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B3::PEST
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFD7

    Plasmid

    #73916

    Purpose

    gRNA vector for AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    tRNA:hAsCpf1-gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINMSYB

    Plasmid

    #41118

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOmnibac_ZZ_linker_ybbR_Tau

    Plasmid

    #159717

    Purpose

    Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Microtubule-associated protein tau (MAPT Human)
    

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Expression

    Insect

    Promoter

    Act5c

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUASattB-PKA-SPARK

    Plasmid

    #106922

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Expression

    Insect

    Available Since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3-Gateway-DSCP

    Plasmid

    #71506

    Purpose

    flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

    Plasmid

    #55221

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217513

    Purpose

    Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIEx4-Dio-GFP

    Plasmid

    #198278

    Purpose

    Cre reporter for insect cells; double-floxxed-EGFP

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pIEx4-Dio-GFP
    

    Expression

    Insect

    Promoter

    IE1 promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBacI-KDAC6

    Plasmid

    #224346

    Purpose

    Expresses human KDAC6 (HDAC6) in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

    Plasmid

    #199197

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int-BlastR
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

    Plasmid

    #199198

    Purpose

    Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int-G418R
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PGRP-SA

    Plasmid

    #21055

    Depositor

    Johann Deisenhofer

    Article

    Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

    Insert

    PGRP-SA (PGRP-SA Fly)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pFastBac1-LRRK2 WD40

    Plasmid

    #139211

    Purpose

    Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

    Depositor

    Hao Wu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    N-His and MBP

    Expression

    Insect

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. vas-Cas9.874Z

    Plasmid

    #112687

    Purpose

    Express hSpCas9 under vasa promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    vasa

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUAST_NSlmb-vhhGFP4

    Plasmid

    #35575

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Expression

    Insect

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pE5c

    Plasmid

    #17465

    Purpose

    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    ECFP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pattB-QF-hsp70

    Plasmid

    #24368

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QF
    

    Expression

    Insect

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHACK(Gal4)-DONR(T2A-Flp1)

    Plasmid

    #194770

    Purpose

    To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-Flp1
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p{ie1-DsRed::GW,attB}

    Plasmid

    #182173

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Expression

    Insect

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge