4,932 results

1. tubP-FRT>GAL80-6-FRT>

   Plasmid

   #63173

   Purpose

   ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "Flp-Out" GAL80
   

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. T5-pAc5.1 EGFP-EcoRV

   Plasmid

   #206481

   Purpose

   Schneider cell imaging

   Depositor

   Anne Ephrussi

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Tags

   EGFP

   Expression

   Insect

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac HT JS-Rab10wt

   Plasmid

   #135557

   Purpose

   Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Tags

   6xHis tag, TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINE-3C-mCherry

   Plasmid

   #214060

   Purpose

   Expression of fluorescent fusion proteins

   Depositor

   Ray Owens

   Insert

   mCherry
   

   Tags

   Hexahistidine

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-dCLK

   Plasmid

   #33153

   Depositor

   Michael Rosbash

   Article

   McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

   Insert

   CLK (Clk Fly)
   

   Use

   Retroviral

   Tags

   6xHIS and V5

   Expression

   Insect

   Promoter

   Actin 5C

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. IMPT-10314:GABBR2

   Plasmid

   #157851

   Purpose

   HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

   Depositor

   Vadim Cherezov, Raymond Stevens

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABBR2 (GABBR2 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc-sgRNA-Cas9

   Plasmid

   #49330

   Purpose

   Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC18-8XLexAop2-mCD8::GFP

   Plasmid

   #26225

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   mCD8::GFP
   

   Expression

   Insect

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLib6.6

    Plasmid

    #176652

    Purpose

    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

    Plasmid

    #206434

    Purpose

    Vasa 463-661 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Vasa 463-661 (vas Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Mutation

    F504E

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1-PH-mCherry (FspAI) (HK49)

    Plasmid

    #206453

    Purpose

    Expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

    Plasmid

    #206461

    Purpose

    Oskar 139-240 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Oskar 139-240 (osk Fly)
    

    Tags

    PH mcherry

    Expression

    Insect

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1-OST4-mCherry (EcoRV) (XH26)

    Plasmid

    #206475

    Purpose

    Expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBPZpGAL4DBDUw

    Plasmid

    #26233

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    Zip-GAL4DBD
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBPGUw

    Plasmid

    #17575

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Tools for neuroanatomy and neurogenetics

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL1392 TMEM97

    Plasmid

    #157748

    Purpose

    Baculovirus transfer vectors to create a virus expressing human TMEM97

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

    Plasmid

    #200400

    Purpose

    Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

    Depositor

    Daniel Kronauer

    Article

    Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

    Inserts

    Discosoma red fluorescent protein
    Q factor 2
    GCaMP6s
    

    Expression

    Insect

    Promoter

    15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTriEx-mCherry-LOV2

    Plasmid

    #81041

    Purpose

    Mammalian or insect expression for wt LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. R57C10-Flp2

    Plasmid

    #63172

    Purpose

    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    

    Expression

    Insect

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

24. 20XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111544

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson_tdtomato_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. TLR4

    Plasmid

    #20863

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Expression

    Insect

    Mutation

    Contains ectodomain (amino acids 27–631)

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBPnlsLexA::p65Uw

    Plasmid

    #26230

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::p65 (RELA Human)
    

    Expression

    Insect

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pACEBac1-PARP1-Flag-His-WT

    Plasmid

    #111572

    Purpose

    Expresses full length PARP1 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. Tubp-Gal4 in pCaSpeR4

    Plasmid

    #17747

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Tags

    Tubulin 1alpha promoter and hsp70 PolyA

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Tags

    3x-Flag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ac5-STABLE1-neo

    Plasmid

    #32425

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    GFP-T2A-neo
    

    Expression

    Insect

    Promoter

    Actin5c

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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33. pFCU1

    Plasmid

    #165842

    Purpose

    5-fluorocytosine sensitivity marker

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    FCU1
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFBOH-MHL.RING1B

    Plasmid

    #224712

    Purpose

    Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    RING1b (RNF2 Human)
    

    Tags

    6x histidine tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pNos-PE2-attB

    Plasmid

    #149549

    Purpose

    Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    nanos (nos)

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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36. pTriEx-NLyn-Venus-Zdk1

    Plasmid

    #81042

    Purpose

    mammalian expression of plasma-membrane anchored Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, NLyn, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTriEx-mCherry-LOV2 V416I

    Plasmid

    #81045

    Purpose

    mammalian expression of LOV V416I

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416I
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416I

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 13xLexAop2 IVS sfGFP1-10

    Plasmid

    #221834

    Purpose

    Plasmid for generating 13xLexAop2 IVS sfGFP1-10 with attP/attB integration. It carries the attB sequence.

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    sfGFP1-10
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge