5,038 results
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Plasmid#42076DepositorAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only
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pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASattB-ERK-SPARK
Plasmid#106923PurposeExpress ERK-SPARK, an activity reporter of ERK in DrosophilaDepositorInsertERK-SPARK
ExpressionInsectAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX1
Plasmid#89310PurposeExpresses HA tagged syntaxin 1 in Drosophila cellsDepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
TagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4DBD-3xP3-RFP
Plasmid#127564PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4DBD
UseCRISPRExpressionInsectAvailable SinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS1
Plasmid#60784PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS1
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2Available SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS ChrimsonR mCherry_tr
Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>mCD8-GFP
Plasmid#24361DepositorInsertmCD8-GFP
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusExpressionInsectPromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#214139PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-3xHA-V5-RpL10Ab
Plasmid#125223Purpose3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expressionDepositorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N116C-G193C
Plasmid#162580PurposeExpresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationAsparagine 116 was mutated to Cysteine and Glycin…PromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-7m1-QFBDADM1-hsp70
Plasmid#46116DepositorInsertQF#7m1
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK23
Plasmid#72851Purposetemplate for C-terminal eGFP tagging, Puromycin-resistanceDepositorInserteGFP
ExpressionInsectPromotercopiaAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmFMR1_B
Plasmid#145920PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
ExpressionInsectMutationTwo amino acid mutations (T214I and N501S) and th…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-Diaphanous-GFP
Plasmid#24287DepositorAvailable SinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
10xUAS-GFP-DD
Plasmid#104989PurposeExpresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized GFP (GFP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)
Plasmid#172066PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC82
Plasmid#118862Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant proteinDepositorInsertNcoI-NotI-ccdB-AscI-stop-HindIII cassette
ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPHK1
Plasmid#73260PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Cdc42
Plasmid#101681Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et alDepositorInsertCdc42
Tags6xHis and FRET Antenna (mCerulean-cpVenus)ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-mCherry-Patronin
Plasmid#31753DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCaryP
Plasmid#18945DepositorInsertphiC31 attP
ExpressionInsectAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Consensus
Plasmid#176664PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pActPL-VP16AD
Plasmid#15305DepositorInsertVP16AD-ZIP+
ExpressionInsectAvailable SinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
TH0901-pOCC177-FUS-wt_opt(Nhe)
Plasmid#221885PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
Tags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationwtPromoterPH promoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
NHR1+2-Flag pBacPAK8-3
Plasmid#17324DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
PGRP-SA
Plasmid#21055DepositorAvailable SinceSept. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3-GFP1-10
Plasmid#138398PurposeExpresses split GFP fragment (GFP1-10)DepositorInsertGFP1-10
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
10XUAS-flp-DD
Plasmid#124777PurposeExpresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized FLP (FLP-DD)
TagsDDExpressionInsectAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL
Plasmid#83342Purposefor Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G
Plasmid#219971PurposeeIF4G protein purificationDepositorAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only