4,932 results

1. pAWH-Rluc-let-7-A114-N40-HhR

   Plasmid

   #50555

   Purpose

   Expression of luciferase reporter mRNA in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

   Insert

   Luciferase-let7-A114-N40-HhR
   

   Use

   Luciferase

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac FPSC

   Plasmid

   #1926

   Depositor

   Robert Kingston

   Article

   Francis et al Mol Cell 2001 Sep;8(3):545-56.

   Insert

   posterior sex combs (Psc Fly)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLib6.6B

   Plasmid

   #176666

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINH

   Plasmid

   #53537

   Purpose

   pTriex-based vector with signal sequence and N-his tag for protein expression

   Depositor

   Ray Owens

   Type

   Empty backbone

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRB36

   Plasmid

   #72861

   Purpose

   template base vector for N-terminal tagging, constitutive Blasticidin resistance

   Depositor

   Klaus Foerstemann

   Article

   Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

   Insert

   base vector for introduction of tags
   

   Expression

   Insect

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Expression

   Bacterial and Insect

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-U63-QtgRNA2.1-8R

   Plasmid

   #170514

   Purpose

   gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGS-FCER1G-1

   Plasmid

   #109192

   Purpose

   Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   Human full-length FCER1G, codon-optimized (FCER1G Human)
   

   Expression

   Insect

   Promoter

   p10

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIE-C-hα2M-H6

    Plasmid

    #128494

    Purpose

    Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (R787-N1473) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Mutation

    contains R787-N1473

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. hs-calx1

    Plasmid

    #1740

    Depositor

    Craig Montell

    Article

    Wang et al Neuron 2005 Feb 3;45(3):367-78.

    Insert

    calx1 (Calx Fly)
    

    Expression

    Insect

    Mutation

    N/A

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. hs-calx2

    Plasmid

    #1741

    Depositor

    Craig Montell

    Article

    Wang et al Neuron 2005 Feb 3;45(3):367-78.

    Insert

    calx2 (Calx Fly)
    

    Expression

    Insect

    Mutation

    N/A

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. 892 pFastBacHTb PTEN

    Plasmid

    #10938

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

    Plasmid

    #169369

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039653

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacPAK8-ElonginC

    Plasmid

    #29505

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Elongin C (ELOC Human)
    

    Expression

    Insect

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pBP-p65AD::CaM

    Plasmid

    #64719

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    p65AD::CaM
    

    Expression

    Insect

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac K-Ring1A

    Plasmid

    #1994

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Ring1A (Ring1 Mouse)
    

    Expression

    Insect

    Mutation

    Kozak before start codon

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pJC-TALE-hairy

    Plasmid

    #46145

    Depositor

    David Stern

    Article

    Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

    Type

    Empty backbone

    Tags

    Hairy Repression Domain

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT-Mad2-GFP

    Plasmid

    #24284

    Depositor

    Ron Vale

    Article

    Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

    Insert

    Mad2 (mad2 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pUASP YFP Rab4 DN

    Plasmid

    #37691

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab4 (Rab4 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S22N (dominant negative)

    Promoter

    GAL4

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUAST-attB-Flag-dTSC2 WT

    Plasmid

    #111807

    Purpose

    Insect Expression of dTSC2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

    Insert

    dTSC2 (gig Fly)
    

    Tags

    3xFlag

    Expression

    Insect

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRB37

    Plasmid

    #72869

    Purpose

    template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    Strep - His8 - Strep -TEV tag
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti PUb

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUASP dsRed Rab11 CA

    Plasmid

    #44830

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q70L (constitutive active)

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. Hy_pMT Laccase2 MCS-WT Split GFP

    Plasmid

    #69891

    Purpose

    Expresses a GFP circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    GFP, laccase2 (stw Fly)
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid

    #111862

    Purpose

    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDN(1-400)-GFP (Bicd2 Mouse)
    

    Tags

    8xHis tag, GFP, and ZZ tag

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmCD8-GFP,y+

    Plasmid

    #24350

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Yellow gene
    

    Tags

    CD8-GFP

    Expression

    Insect

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ArpC5_pLib

    Plasmid

    #173682

    Purpose

    ArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC5 (ARPC5 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ArpC3-FLAG_pLib

    Plasmid

    #173680

    Purpose

    ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC3 (ARPC3 Human)
    

    Tags

    TEV cleavage site and FLAG tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pF3BGX-T2A-GAL80

    Plasmid

    #138396

    Purpose

    RMCE vector for generating C-terminal T2A-GAL80 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL80
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Tags

    T2A-GFP

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL2-ptc-lu

    Plasmid

    #37678

    Depositor

    Philip Beachy

    Article

    Chen et al Cell. 1999 Aug 6;98(3):305-16.

    Insert

    ptc promoter (−758 to +130)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    ptc

    Available Since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pGB-08-09-BECN1

    Plasmid

    #187991

    Purpose

    Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325

    Depositor

    Sascha Martens

    Insert

    BECN1 (BECN1 Human)
    

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUAST-HA-dAtg13

    Plasmid

    #111802

    Purpose

    Insect Expression of dAtg13

    Depositor

    Jun Hee Lee

    Article

    Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

    Insert

    dAtg13 (Atg13 Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

    Plasmid

    #146503

    Purpose

    Insect Expression of DmDCP1_327-372

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DmDCP1_327-372 (Dcp1 Fly)
    

    Expression

    Insect

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pattB-nsyb-MKII::nlsLexADBDo

    Plasmid

    #64725

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKII::nlsLexADBDo
    

    Expression

    Insect

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge