75,820 results

1. pMSCVII

   Plasmid

   #162750

   Purpose

   MSCV backbone

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. psfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164477

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSFFV_sfCherry3V(1-10)

   Plasmid

   #117483

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3V(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3V(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   6 substitutions (K45R, G52D, L83W, T106A, K182R, …

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

   Plasmid

   #197989

   Purpose

   For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   HSALNT0217290
   

   Use

   Lentiviral

   Tags

   lac

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4xmts-mScarlet-I

   Plasmid

   #98818

   Purpose

   In vivo visualization of mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   mts
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PBRM1

   Plasmid

   #65387

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6M GLUT3

   Plasmid

   #194139

   Purpose

   Expresses GLUT3 protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC2A3 (SLC2A3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-CAG-gate-FLAG-IRES-GFP

   Plasmid

   #107398

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-KIT-V5/HIS

   Plasmid

   #201986

   Purpose

   expression of human KIT receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   KIT receptor tyrosine kinase (KIT Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-PKAcat-mCherry

    Plasmid

    #181841

    Purpose

    mCherry-tagged PKA catalytic subunit for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PKAcat-mCherry (PRKACA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-mCherry

    Plasmid

    #193293

    Purpose

    episomal vector expressing mCherry fluorescent protein

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV-SS-antiFLAGscFv-TM

    Plasmid

    #198303

    Purpose

    Displays anti-FLAG scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiFLAGscFv-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IB-HaloTag7-mGFP

    Plasmid

    #184903

    Purpose

    Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    monomeric EGFP
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. M_ST1n_VPR

    Plasmid

    #63799

    Purpose

    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    ST1-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2 GW delCMV

    Plasmid

    #29496

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TSHR-Tango

    Plasmid

    #66518

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TSHR (TSHR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. hBACCS2-IRES-mCherry

    Plasmid

    #72892

    Purpose

    Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    hBACCS2-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1+_Lambda-N-HA-Peptide

    Plasmid

    #92005

    Purpose

    Expresses Lambda-N-HA peptide

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Lambda-N-HA peptide
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-CAG-jGCaMP8m-WPRE

    Plasmid

    #179255

    Purpose

    AAV-mediated expression of GCaMP8m under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ISceI-GR-RFP

    Plasmid

    #17654

    Depositor

    Tom Misteli

    Article

    Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

    Insert

    I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C655G mutation in the glucocorticoid receptor (se…

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SOST/pcDNA3.1+

    Plasmid

    #10842

    Depositor

    Xi He

    Article

    Semenov et al J Biol Chem. 2005 Jul 22. 280(29):26770-

    Insert

    SOST (SOST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CKIIa-stGtACR2-FusionRed

    Plasmid

    #105669

    Purpose

    Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde and AAV1

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pcDNA3-TLR10-YFP

    Plasmid

    #13643

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 10 (TLR10 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF1708

    Plasmid

    #143970

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPP1R13L (PPP1R13L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLJM1-Tmem192-mRFP-3xHA

    Plasmid

    #134631

    Purpose

    bait for affinity-isolation of lysosomes

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3xHA, TEV, and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and Murine Gclc ARE

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTipi2.1

    Plasmid

    #225877

    Purpose

    Allows for expression of recombinant antibodies

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSCVII-tdTomato

    Plasmid

    #162752

    Purpose

    Retroviral (MSCV) expression of td Tomato fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    tdTomato
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiGuide-puro-NLS-mCherry

    Plasmid

    #185474

    Purpose

    Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Flag-GFP11-pDEST-C

    Plasmid

    #118367

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    GFP11-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBbi 41BBL-CD86

    Plasmid

    #183244

    Purpose

    Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    CD86, 41BBL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge