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Mammalian Expression

75,750 results

Showing: 901 - 950 of 75750 results

  1. TFORF1898

    Plasmid
    #144116
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PATZ1 (PATZ1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144116-plasmid-map-sequence-id-396183

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  3. pZac2.1 gfaABC1D-cyto-GCaMP8f

    Plasmid
    #186050
    Purpose
    AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186050-plasmid-map-sequence-id-370166

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  5. TFORF2610

    Plasmid
    #144136
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXA2 (FOXA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    144136-plasmid-map-sequence-id-386562

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  8. mRuby-Golgi-7

    Plasmid
    #55865
    Purpose
    Localization: Golgi Complex, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. TFORF2640

    Plasmid
    #144406
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CIC (CIC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144406-plasmid-map-sequence-id-353329

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  12. mEos3.2-PSD95-N-14

    Plasmid
    #57480
    Purpose
    Localization: Membrane Scaffold, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    PSD95
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. TFORF3198

    Plasmid
    #144674
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    OVOL2 (OVOL2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144674-plasmid-map-sequence-id-350531

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  20. dCas9-GCN5 FL

    Plasmid
    #179552
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179552-plasmid-map-sequence-id-354751

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  22. HTR1F-Tango

    Plasmid
    #66408
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR1F (HTR1F Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  31. PIK3CA-SmBiT Fusion Vector

    Plasmid
    #237093
    Purpose
    Express PIK3CA-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Early Access Program
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237093-plasmid-map-sequence-id-473322

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  32. PIK3CA-LgBiT Fusion Vector

    Plasmid
    #237094
    Purpose
    Express PIK3CA-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Early Access Program
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237094-plasmid-map-sequence-id-473323

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  33. SmBiT-MDM2 Control Vector

    Plasmid
    #237037
    Purpose
    Express SmBiT-MDM2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Early Access Program
    Has Service
    DNA
    Insert
    MDM2 (MDM2 Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237037-plasmid-map-sequence-id-471894

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  34. LgBiT-p53 Control Vector

    Plasmid
    #237036
    Purpose
    Express LgBiT-p53 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Early Access Program
    Has Service
    DNA
    Insert
    p53 (TP53 Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237036-plasmid-map-sequence-id-471893

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  36. TFORF0591

    Plasmid
    #142654
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXO4 (FOXO4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142654-plasmid-map-sequence-id-462879

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  37. pCMV-PE2-P2A-PuroR

    Plasmid
    #196962
    Purpose
    Expresses PE2 with puromycin resistance
    Depositor
    Leopold Parts
    Insert
    PE2-P2A-Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    196962-plasmid-map-sequence-id-440213

    Enlarge

  39. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

    Plasmid
    #204696
    Purpose
    This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of the amino acids 355-505 of NUT
    Promoter
    CMV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204696-plasmid-map-sequence-id-405172

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  40. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

    Plasmid
    #204695
    Purpose
    This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-NUT(MIN) (BRD4, NUTM1 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of majority of NUT, retaining only amino…
    Promoter
    CMV
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204695-plasmid-map-sequence-id-405171

    Enlarge

  41. TFORF2329

    Plasmid
    #143186
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CIZ1 (CIZ1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143186-plasmid-map-sequence-id-397832

    Enlarge

  42. TFORF0676

    Plasmid
    #142191
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM16 (PRDM16 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142191-plasmid-map-sequence-id-397696

    Enlarge

  43. TFORF1024

    Plasmid
    #144268
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXI3 (FOXI3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144268-plasmid-map-sequence-id-391493

    Enlarge

  44. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

    Enlarge

Showing: 901 - 950 of 75750 results