78,292 results

1. pIRES proHB-EGF WT

   Plasmid

   #11602

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Expression

   Mammalian

   Available Since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

2. 2C-EGFP-t2a-mCD4

   Plasmid

   #216213

   Purpose

   Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

   Depositor

   Michelle Percharde

   Article

   Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

   Insert

   CD4 (Cd4 Mouse)
   

   Tags

   GFP-T2A

   Expression

   Mammalian

   Mutation

   CD4 transmembrane domain only

   Promoter

   MERVL

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Ezrin-C-14

   Plasmid

   #55042

   Purpose

   Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   NM_003379.4 (EZR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 Ash2L full

   Plasmid

   #15548

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Ash2L (ASH2L Human)
   

   Expression

   Mammalian

   Mutation

   R622H (Please see depositor comments below)

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRUPATH Triple Gagust

   Plasmid

   #196054

   Purpose

   Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

   Plasmid

   #208697

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO V5 HSPA1A

   Plasmid

   #19510

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 1A (HSPA1A Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS006

    Plasmid

    #211506

    Purpose

    AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-NIR-GECO2

    Plasmid

    #159603

    Purpose

    AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Insert

    NIR-GECO2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK_neo_3xflag_CHEK2

    Plasmid

    #136536

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CHEK2 (CHEK2 Human)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    June 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIS1_Opt_Renilla

    Plasmid

    #210571

    Purpose

    Expresses Optimal Renilla luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Optimal Renilla
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    HSV TK; T7

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 NRF2

    Plasmid

    #118708

    Purpose

    entry clone for NRF2

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    NRF2
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCDH-SIRT6

    Plasmid

    #102325

    Purpose

    Human SIRT6 for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1-nv5-ER beta

    Plasmid

    #22770

    Depositor

    Donald McDonnell

    Article

    Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

    Insert

    estrogen receptor beta (ESR2 Human)
    

    Tags

    v5

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. mNeonGreen-Sec31A

    Plasmid

    #226582

    Purpose

    Encodes Sec31A protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

    Insert

    Sec31A (Protein transport protein) (SEC31A Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAS9PBKS

    Plasmid

    #68371

    Purpose

    CbAkt promoter driven Cas9-2A-GFP (from px458)

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    Cas9
    

    Tags

    2A-EGFP

    Expression

    Mammalian

    Promoter

    CbAkt

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPRIME-TET-GFP-FF3

    Plasmid

    #11662

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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