74,914 results

1. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVSV-G

   Plasmid

   #138479

   Purpose

   Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   VSV-G envelop protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-GFP11-Clathrin light chain

   Plasmid

   #70217

   Purpose

   Expresses GFP11-clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11-Clathrin light chain (CLTA Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GFP(1-10)

   Plasmid

   #70219

   Purpose

   Expresses GFP(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-CFP

   Plasmid

   #13030

   Purpose

   Mammalian expression vector for cloning your gene fused to CFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   cyan fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ClpX

   Plasmid

   #234733

   Purpose

   Human-codon optimized E. coli ClpX in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ClpX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaV1.2/pcDNA3.1~ Human

   Plasmid

   #140576

   Purpose

   Expression in mammallian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

   Insert

   human CaV1.2 (CACNA1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC407 - pAAV EF1a eGFP

    Plasmid

    #60058

    Purpose

    An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    enhanced Green Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX313-Renilla luciferase

    Plasmid

    #118016

    Purpose

    Constitutive expression of Renilla luciferase

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-NINJ1

    Plasmid

    #201180

    Purpose

    Transient expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-STAT3-Y705F

    Plasmid

    #74434

    Purpose

    Expression of STAT3-Y705F

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutations E16K, Y705F

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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