76,374 results
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pLenti CMV/TO SV40 small + Large T (w612-1)
Plasmid#22298DepositorInsertSV40 small + Large T antigen
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-pCAG eGFP T2A SP HALO HAPLN1
Plasmid#228200PurposeAAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoterDepositorInsertHAPLN1 (Hapln1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterconstitutive CAG promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BLM
Plasmid#110299PurposeMammalian expression of a EGFP-tagged full length Bloom's syndrome helicaseDepositorInsertBLM (Bloom's Syndrome) helicase (BLM Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81
Plasmid#100157PurposeExpresses MUS81DepositorInsertMUS81 (MUS81 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
phU1-PspCas13b-non-targeting (pEZ36)
Plasmid#229053PurposeCloning backbone for PspCas13b crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest BlastR R1-R2
Plasmid#84574PurposeGateway destination vector expressing Blasticidin resistance under a PGK promoterDepositorTypeEmpty backboneUseLentiviral; GatewayTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH
Plasmid#105553PurposeAAV expression of Cre from hSyn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti SpBsmBI sgRNA Hygro
Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Zeo-dTAG
Plasmid#207705PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26
Plasmid#232929PurposeTargeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.DepositorInsertOsTir1(F74G)
UseMouse TargetingTagsExpressionMammalianMutationchanged Phenylalanine 74 to GlycinePromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro-EGFP
Plasmid#219795PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stat3-C Flag pRc/CMV
Plasmid#8722DepositorInsertStat3-C (Stat3 Mouse)
UseTagsFlagExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders…PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEF-GFP
Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha Flag pRc/CMV
Plasmid#8691DepositorInsertSTAT1 alpha (STAT1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)
Plasmid#185913PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DAAO
Plasmid#60344PurposeYeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.DepositorInsertDAAO
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-hPGK-Blast-2A-STING-TurboID
Plasmid#204713PurposeExpresses C-terminal TurboID tagged human STING for proximity ligation.DepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationPromoterhPGKAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3G
Plasmid#14748DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HyPer7-MEM
Plasmid#136465PurposeMammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsFarnselation tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
V5-miniTurbo-NES_pCDNA3
Plasmid#107170Purposeexpresses V5-tagged miniTurbo in the mammalian cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-OTUD4
Plasmid#22594DepositorInsertOTUD4 (OTUD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRXRα
Plasmid#135910PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 PromoterDepositorInsertHuman retinoid X receptor- alpha (RXRA Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(wt)
Plasmid#87929PurposeExpresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationNonePromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsXRN1
Plasmid#66596Purposeexpresses lambdaN-HA-tagged XRN1 in mammalian cellsDepositorInsertXRN1 (XRN1 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mEsrra
Plasmid#173152Purposemouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cellsDepositorInsertORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
UseTagsHisExpressionMammalianMutationWild typePromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti
Plasmid#106280PurposeA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-AKAP95-FRB
Plasmid#138254PurposeFull-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertAKAP95-FRB (AKAP8 Human)
UseTagsFRB rapamycin binding domain and HA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-SOX9
Plasmid#170703Purposedoxycycline-inducible overexpression of SOX9DepositorInsertSOX9 (SOX9 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
UseTagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE
Plasmid#180280PurposeThe CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.DepositorInsertCasMINI sgRNA (backbone) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only
