79,276 results

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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TET-MKOS

Plasmid

#20959

Purpose

piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pLV6-Bmal-luc

Plasmid

#68833

Purpose

Expression of Bmal1-luc in mammalian cells

Depositor

Steven Brown

Insert

Bmal1 regulatory sequences (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.22_mCherry-MS2x8

Plasmid

#205537

Purpose

Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 TrkA

Plasmid

#15002

Depositor

Raymond Birge

Article

Yano et al J Neurosci Res. 2000 Feb 1. 59(3):356-64

Insert

TrkA (NTRK1 Human)

Expression

Mammalian

Mutation

V289L

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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mCherry-DFCP1

Plasmid

#86746

Purpose

N-terminal mCherry-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human DFCP1 (ZFYVE1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-2xCL-YFP-Vpr

Plasmid

#105215

Purpose

Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

Depositor

Gregory Melikyan

Article

Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

Insert

Vpr

Tags

PC2x (SQNY/IRKVL), eYFP, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-V

Plasmid

#27290

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha V (ITGAV Human)

Expression

Mammalian

Mutation

Isoform 2

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-N E3gp 19k

Plasmid

#38049

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

E3 gp19K

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 SARS-CoV-2 S D614G

Plasmid

#158075

Purpose

Expresses SARS-CoV-2 spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FUS_FLI1

Plasmid

#205822

Purpose

Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

FUS_FLI1 (FUS, FLI1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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m6A-Tracer-NES

Plasmid

#159607

Purpose

Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

m6A-Tracer-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSV40_HA-mTarbp2

Plasmid

#120539

Purpose

protein expression

Depositor

Petr Svoboda

Insert

TARBP2 (Tarbp2 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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eIF2a 3

Plasmid

#21809

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Mutation

S52D

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pRVdG-N-P-M-nucBFP-EGFP-L

Plasmid

#52485

Purpose

Expresses nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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