78,616 results

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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GluN2A (px330:2-guideCas9)

Plasmid

#169428

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0095

Plasmid

#142145

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZEB2 (ZEB2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ER alpha

Plasmid

#28230

Purpose

Mammalian expression of Estrogen Receptor alpha fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Insert

Estrogen Receptor alpha (ESR1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-Eco

Plasmid

#35617

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

Ecotropic MLV envelope

Expression

Mammalian

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO

Plasmid

#237295

Purpose

Empty Sleeping Beauty XPRESSO vector.

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (C-flag)

Plasmid

#156420

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-KRT5 hygro

Plasmid

#58493

Purpose

mammalian expression of mRFP1-tagged human KRT5

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

KRT5 (KRT5 Human)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

Plasmid

#141368

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLX304

Plasmid

#25890

Purpose

3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-TET2BlastR-Cas9-TagBFP

Plasmid

#236726

Purpose

Dox Inducible Cas-TagBFP expression

Depositor

David Bersten

Insert

Cas9-TagBFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mcherry-DN KASH

Plasmid

#125553

Purpose

mcherry labeled DN-KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K29R

Plasmid

#17602

Purpose

Mammalian expression of HA tagged ubiquitin K29R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29R; V5 deleted

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pM162

Plasmid

#173221

Purpose

Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Inserts

ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Article

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-Flex-GFP-Fishell_6

Plasmid

#83895

Purpose

Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

Insert

eGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

Plasmid

#141388

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-TetOne-Puro-LUC

Plasmid

#218630

Purpose

This plasmid contains luciferase under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LUC (LOC116160065 )

Use

Lentiviral, Luciferase, and Synthetic Biology

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM201

Plasmid

#173179

Purpose

dCas9-24xGCN4

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-24xGCN4

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2476

Plasmid

#144030

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFHX2 (ZFHX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hUtx-IRES-Hyg

Plasmid

#122374

Purpose

Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hUtx-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc3-Cul7

Plasmid

#20695

Depositor

Yue Xiong

Article

Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

Insert

cullin 7 (CUL7 Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0459

Plasmid

#142266

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB5 (CREB5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0461

Plasmid

#142267

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB5 (CREB5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ149

Plasmid

#41951

Depositor

Stephen Elledge

Article

Zhang et al Cell. 2006 Aug 11;126(3):529-42.

Insert

ubiquitin specific peptidase 28 (USP28 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

C171A

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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78,616 results