78,292 results

1. pCMV mClover3-Galectin-3

   Plasmid

   #215376

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP8s

   Plasmid

   #162371

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-Ostir-neo

   Plasmid

   #161973

   Purpose

   Expresses Ostir and G418 resistance gene in mammalian cells

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Ostir-3xHA-p2a-neoR
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

   Plasmid

   #216244

   Purpose

   Express AtAFB2.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A-mCherry
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh and U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1477 pcDNA3 flag HA Akt1

   Plasmid

   #9021

   Depositor

   William Sellers

   Article

   Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   HA and flag

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-rat Nrxn1alpha

   Plasmid

   #58266

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1alpha

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1alpha (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HA-NLGN3 WT

   Plasmid

   #59318

   Purpose

   Expression plasmid of HA-tagged wildtype human Neuroligin 3

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN3 (NLGN3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMPRA1

    Plasmid

    #49349

    Purpose

    Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

    Plasmid

    #140534

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-NLS TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid

    #139989

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CMV.Luc.IRES.EGFP.SV40

    Plasmid

    #105533

    Purpose

    AAV expression of Luciferase and EGFP from CMV promoter

    Depositor

    James M. Wilson

    Insert

    Luciferase
    

    Use

    AAV and Luciferase

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hAdipoq-GFP

    Plasmid

    #176215

    Purpose

    Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

    Depositor

    Yu-Hua Tseng

    Article

    Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA6-N-Myc-SCAP

    Plasmid

    #134285

    Purpose

    Expresses Myc-tagged SCAP in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SCAP (Scap, Scap Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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