74,946 results

1. pRK5-myc-Miro1

   Plasmid

   #47888

   Purpose

   Expresses myc tagged Miro1

   Depositor

   Pontus Aspenström

   Article

   Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-BSD-tet-mEGFP-MIB1

   Plasmid

   #140241

   Purpose

   Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   Tet-inducible mEGFP-MIB1 (MIB1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-58: NUP153-mEGFP

   Plasmid

   #114407

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153

   Depositor

   Allen Institute for Cell Science

   Insert

   NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX-puro_G-CEPIA1er

   Plasmid

   #164590

   Purpose

   Stable integration and expression of G-CEPIA1er in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   G-CEPIA1er
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN.AAV.CMVs.Pl.Cre.rBG

   Plasmid

   #105537

   Purpose

   AAV expression of Cre from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV minimal

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV-CFP

   Plasmid

   #25998

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-CFP (H2BC21 A. victoria, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

   Plasmid

   #187588

   Purpose

   Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NLS
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG-Hc∆18

    Plasmid

    #84817

    Purpose

    Measles virus hemagglutinin expressing plasmid

    Depositor

    Jakob Reiser

    Article

    Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

    Plasmid

    #182925

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mcherry-DN KASH

    Plasmid

    #125553

    Purpose

    mcherry labeled DN-KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    CMV

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBRPB CAG-mCherry-IP

    Plasmid

    #106333

    Purpose

    PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    mCherry-IRES-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-TSC2

    Plasmid

    #15478

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    TSC2 shRNA (TSC2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge