78,616 results
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pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPXL
Plasmid#12257Purpose2nd generation lentiviral transfer plasmidDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsEGFPExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLENTICRISPR V2 sgCGAS_1
Plasmid#241454PurposeDelete CGASDepositorAvailable SinceNov. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-hPGK-Blast-2A-STING-mNeonGreen
Plasmid#227186PurposeLentiviral expression plasmid encoding STING-mNeonGreen under hPGK promoterDepositorAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
SUPV3L1-HA G207V
Plasmid#232349PurposeExpresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tagDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Blast-ARL13B
Plasmid#227278PurposeDonor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)DepositorInsertARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ARL13B
Plasmid#227276PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.DepositorInsertsgRNA Targeting C-terminus of ARL13B (ARL13B Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_SigP:NLuc
Plasmid#197263PurposeCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…ExpressionMammalianAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-HsSAS6
Plasmid#136790PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tagDepositorInsertSNAP-SAS6 (SASS6 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNR2F1.1.0-gDNA
Plasmid#112484PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_EV
Plasmid#183116PurposeEmpty vector control for pLenti_EF1a constructsDepositorInsertSmall multiple cloning site
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-AHPH-DM
Plasmid#71368PurposeLocation sensor for RhoA-GTPDepositorInsertC terminus of Anillin (ANLN Human)
TagsEGFPExpressionMammalianMutationA740D and E758KPromoterCMVAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
TagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLI_TNF
Plasmid#171179Purposeinducible expression of human TNFDepositorInserthuman TNF
UseLentiviralTagsnoneExpressionMammalianPromotertight TRE promoterAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]
Plasmid#247251PurposeMeasure ISCro4 activity via luciferase expressionDepositorInsertpNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
ExpressionMammalianAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
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Orai1 CFP
Plasmid#19757DepositorAvailable SinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-MT2A
Plasmid#11613DepositorAvailable SinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
Plenti-ARE-HSVTK-luc
Plasmid#161789PurposeExpresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markersDepositorInsertsLuciferase-T2A-HSV-TK
mCherry P2A blasticidin
UseLentiviral and LuciferaseExpressionMammalianPromoterEF1a and Murine Gclc AREAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin_psychLight2
Plasmid#163909PurposeTo generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoterDepositorHas ServiceAAV9InsertpsychLight2
UseAAVTagsFlag tagExpressionMammalianPromoterSynapsinAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-null
Plasmid#200182PurposeExpresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 siteDepositorInsertAAV9 Cap w/ nonsense mutation at VR8
UseAAVExpressionMammalianMutationReplaced amino acids 586-592 in Cap with nonsense…Available SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxExpressionMammalianAvailable SinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ArchT-BFP2-TSERex
Plasmid#123312PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertArchT-BFP2
ExpressionMammalianPromoterCAGAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pur-mEGFP
Plasmid#186329PurposeFluorescent reporter systemDepositorInsertmEGFP
ExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDual-IN-SMN1
Plasmid#215721Purposedual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1DepositorInsertSMN1 EXON 7 (SMN1 Human)
ExpressionMammalianAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-mgU2-47-Mango II
Plasmid#127586PurposeExpresses mgU2-47-Mango II scaRNA from a mU6 promoterDepositorInsertmgU2-47 scaRNA with Mango II tag
ExpressionMammalianPromotermodified U6Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only
