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Showing: 721 - 760 of 4625 results
  1. nCas9-PPE

    Plasmid
    #140445
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pYPQAT04

    Plasmid
    #223376
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Insert
    ZmUbi-SpCas9-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQ269E2

    Plasmid
    #164721
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing
    Depositor
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…
    Expression
    Plant
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. EFR WT

    Plasmid
    #102413
    Purpose
    fluorescent tagging. Plant expression of EFR WT
    Depositor
    Insert
    EFR (EFR Mustard Weed)
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Promoter
    35S
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pFH103

    Plasmid
    #128208
    Purpose
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Insert
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pUPD2:35S-TMV-Rep-MP

    Plasmid
    #106217
    Purpose
    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)
    Depositor
    Insert
    Infectious clone of Tobacco mosaic virus, 5' part
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. GB4070

    Plasmid
    #187811
    Purpose
    Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
    Depositor
    Insert
    copper-inducible dCasEV2.1
    Expression
    Plant
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pGH00.0126

    Plasmid
    #64257
    Purpose
    Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation
    Inserts
    EGFP
    neomycin phosphotransferase II
    Use
    Binary agrobacterium vector for plant transformat…
    Expression
    Plant
    Promoter
    E12-omega
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 721 - 760 of 4625 results