4,297 results
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Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pORFE0010
Plasmid#112074Purpose3xmVenus module CTDepositorInsert3xmVenus
UseTagsExpressionPlantMutationSer to Thr at amino acid 63 and Phe to Leu at pos…PromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLCS101
Plasmid#209355PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mVenus fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH-PABE-7-sgRNA
Plasmid#115621PurposeTargeted A to G in riceDepositorInsertwtTadA-TadA7.10-nCas9-3*NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpG-PPE
Plasmid#170130PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9-SpG(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…Promotermaize Ubiquitin-1Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOT2-poly-cis
Plasmid#141177PurposeTo clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoterDepositorTypeEmpty backboneUseRNAi and Synthetic BiologyTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnI/trnA
Plasmid#123579PurposeMoChlo kitDepositorInsertN. tabacum trnI/trnA integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAXY0007
Plasmid#205576PurposeNpuDnaE(C)-Glucos(232-500)DepositorInsertNpuDnaE(C)-Glucosyltransferase
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0006
Plasmid#205575PurposeGlucos(1-231)-NpuDnaE(N)DepositorInsertsGlucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-SpRY-Act3.0
Plasmid#178957PurposeIt consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertSpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEr
Plasmid#61208PurposeFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-mCherry-BCP
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-B/c-AtMIR173
Plasmid#137885PurposePlant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.DepositorInsertsAtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos
UseTagsExpressionPlantMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL2013
Plasmid#172182PurposeA CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.DepositorInsertmCherry
UseCRISPRTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC10
Plasmid#104784PurposepAH595-gRNA 2xplex entry cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RR
Plasmid#108860PurposeLbCpf1 Gateway entry plasmid with G548R and K611R mutationsDepositorInsertLbCpf1-RR (LbCpf1 with G548R and K611R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R and K611R, Cpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB303
Plasmid#68631PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LS-L-11
Plasmid#197719PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LSL in c-sense with GG expansion to v-sense ORF position
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCJJ125
Plasmid#117515PurposeCas9_3 (with intron) Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_3(intron):GCTT:BsaI
UseCRISPRTags2xFLAG and NLSExpressionPlantMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX024
Plasmid#126242PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf03035g01006.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE161
Plasmid#79453Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-AUTr
Plasmid#61204PurposeFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoterDepositorInsertLC3(ATG8)
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB-cHyPer2
Plasmid#84728PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxideDepositorInsertHyPer2
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRETtv-2in1-CN
Plasmid#105119PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmTRQ2 and mVenusExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S-A3A-PBE
Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
UPG
Plasmid#161003Purposeubiquitous expression of PCP-GFPDepositorInsertPCP-GFP
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB229
Plasmid#68620PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB114
Plasmid#68568PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
UPmCh
Plasmid#161004Purposeubiquitous expression of PCP-mCherryDepositorInsertPCP-mCherry
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_nCas9-PmCDA_Hyg_ALS
Plasmid#91693PurposeMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-CC
Plasmid#105115PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmEGFP/mCherryExpressionPlantMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHairyRed (double 35s removed)
Plasmid#69710PurposeFluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modifiedDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRETtv-2in1-CC
Plasmid#105116PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmTRQ2/mVenusExpressionPlantMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
JAB2240
Plasmid#190359PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)DepositorInsertproG1090::PhlF-NLS-19Sterm
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB521
Plasmid#74863PurposeGateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZA72
Plasmid#174783PurposeIn planta gene expressionDepositorInsertGG_XopJ4, Kmr plant selection marker
UseTagsExpressionPlantMutationWT + STOP codonPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-RedFLuc-9
Plasmid#197738PurposePhytobrick (MoClo) Level 0 PartDepositorInsertRedF Luciferase (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE392
Plasmid#149282PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
UMsfG
Plasmid#161005Purposeubiquitous expression of MCP-sfGFPDepositorInsertMCP-sfGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only