4,545 results

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pDIRECT_21A

Plasmid

#91128

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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p32Lb

Plasmid

#158154

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB543

Plasmid

#74877

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-7

Plasmid

#126055

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL063

Plasmid

#124895

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19/Mito-mChe

Plasmid

#183167

Purpose

Construct for transient expression of mitochondrion-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Mito-mCherry

Tags

OsCOX11, Oryza sativa cytochrome c oxidase assemb…

Expression

Plant

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB116

Plasmid

#68570

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pH-AFID-3

Plasmid

#167361

Purpose

For inducing predictable deletions in rice plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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hygII-UT-ABAleon2.1

Plasmid

#106982

Purpose

ABAleon2.1 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Expression

Plant

Promoter

UBQ10

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ284-RVRR

Plasmid

#160327

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a RVRR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N563R, K569V, N573R and K625R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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L1P80

Plasmid

#195941

Purpose

Level 1 pLBD16 reporter : pLBD16-mScarlet-NLS-tRBCS

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pLBD16-mScarlet-NLS-tRBCS

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAP4

Plasmid

#136079

Purpose

Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEL066

Plasmid

#124897

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

3xFlag

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQAT45

Plasmid

#223417

Purpose

T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-LbCas12a-D156R-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
esgRNA_NbPDS

Plasmid

#231147

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting NbPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID-GFP

Plasmid

#209399

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB407

Plasmid

#74801

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHW90004

Plasmid

#222436

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CDS12_mTurquoise2

Plasmid

#136089

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pICSL86977OD

Plasmid

#86180

Purpose

GG compatible CDs acceptor BASTA

Depositor

Mark Youles

Type

Empty backbone

Expression

Plant

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_C-mEGFP

Plasmid

#231279

Purpose

For stably expressing fusion proteins with C terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAB18-GFP

Plasmid

#106984

Purpose

ABA signaling reporter, GFP under the RAB18 promoter

Depositor

Julian Schroeder

Article

Kim et al Curr Biol. 2011 Jun 7;21(11):990-7. doi:

Insert

sGFP

Expression

Plant

Promoter

RAB18

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
35S::GeNL/pCAMBIA1301

Plasmid

#190070

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPL-Gg

Plasmid

#61653

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
lvl0 eCFP CDS

Plasmid

#153397

Purpose

Cyan fluorescent protein coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eCFP CDS

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_211

Plasmid

#91110

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-cTP-TurboID

Plasmid

#209394

Purpose

Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

cTP-3XHA-TurboID

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Tags

Citrine

Expression

Plant

Promoter

ProRUBY

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ144-ZmUbi-tRNA

Plasmid

#158402

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AL12R-HSP101

Plasmid

#161014

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PABE-7-NG

Plasmid

#138142

Purpose

Targeted A to G in rice protoplasts

Depositor

Caixia Gao

Insert

wtTadA-TadA7.10-nCas9-NG-3XNLS

Expression

Plant

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-1-BeYDV-LS-L-11

Plasmid

#197717

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV LSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-1-BeYDV-LRS-L-11

Plasmid

#197718

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

BeYDV LRSL in c-sense with GG expansion to v-sense ORF position

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKPI-MtLAP1

Plasmid

#193521

Purpose

Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

Depositor

Jeremy Murray

Article

Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

Inserts

MtLAP1
DsRed

Expression

Plant

Promoter

ATUBI10 and Medtr8g059790(KPI)

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-TurboID-3XNLS

Plasmid

#209392

Purpose

Transiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-3XNLS

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMU-LEr

Plasmid

#61185

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH114

Plasmid

#128408

Purpose

Level1 ZmUBIp::BAR:NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level1 ZmUBIp::BAR:NOSt (Position 1)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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4,545 results