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Showing: 151 - 200 of 1735 results

  1. pJM656

    Plasmid
    #53086
    Purpose
    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53086-plasmid-map-sequence-id-82567

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  3. T444T-GFP(NCO)

    Plasmid
    #183972
    Purpose
    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP
    Depositor
    David Matus
    Article
    Palmisano et al CourseSource, 2022
    Insert
    GFP (non-codon-optimized)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    183972-plasmid-map-sequence-id-364073

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  7. L4817

    Plasmid
    #1683
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, gfpN, IVSR, gfpGLA, IVSS, IVS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1683-plasmid-map-sequence-id-293

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  13. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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  15. pDD362

    Plasmid
    #137023
    Purpose
    SapTrap HaloTag-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized Halo Tag
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized Halo Tag
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137023-plasmid-map-sequence-id-274041

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  19. pCC324

    Plasmid
    #110165
    Purpose
    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    R61K change made in ERK-KTR CDS
    Promoter
    lin-31 promoter/enhancer
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110165-plasmid-map-sequence-id-235338

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  22. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

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  23. pJH3449

    Plasmid
    #179576
    Purpose
    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    BFP
    Expression
    Worm
    Mutation
    Promoter
    Plgc-55B
    Available since
    March 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    179576-plasmid-map-sequence-id-355663

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  27. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  32. pNMSB84

    Plasmid
    #199309
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199309-plasmid-map-sequence-id-404774

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  39. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  41. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pJH4542

    Plasmid
    #200779
    Purpose
    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    GCaMP6
    Use
    Tags
    mNeptune
    Expression
    Worm
    Mutation
    Promoter
    Ptwk-40
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200779-plasmid-map-sequence-id-412786

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  45. pJW2086

    Plasmid
    #154335
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::GFP::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    154335-plasmid-map-sequence-id-308930

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Showing: 151 - 200 of 1735 results