8,173 results
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pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionYeastAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML104
Plasmid#67638PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52 and pTDH3Available SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
TagsHA and c-mycExpressionYeastAvailable SinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
TagsGFPExpressionYeastAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#49425PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterATG8Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
TagsymScarletIExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-SpHis5
Plasmid#125704PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selectionDepositorInsertymNeonGreen
TagsymNeonGreenExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTurquoise2-Kan
Plasmid#224092PurposeYeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selectionDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEmCitrine-Hygro
Plasmid#44645DepositorInsertyEmCitrine
ExpressionYeastMutationA206RAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSII41K
Plasmid#194522PurposeLow copy vector backbone with G418 selectivity, contains MCSDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastPromoterGAL1Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPIC9K_A2AR_316_V229C_R291A
Plasmid#246917PurposeExpress A2AR mutants in Pichia PastorisDepositorInsertAdenosine A2A receptor (ADORA2A Human)
Tags10xHis and alpha-factor secretion signal, FLAGExpressionYeastMutationtruncated to aa 1-316, V229C, R291APromoterAOX1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-GW
Plasmid#61703PurposeGateway compatible Y2H vector - BDDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleAvailable SinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-GW
Plasmid#61702PurposeGateway compatible Y2H vector - ADDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HaloTag-KanMX6
Plasmid#87029PurposeTag S. pombe genes in C terminal with HaloTagDepositorInsertHaloTag
ExpressionBacterial and YeastAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHA-VRC01-scFv
Plasmid#44874DepositorInsertVRC01 scFv
TagsHA and MycExpressionYeastPromoterGAL1Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pML107
Plasmid#67639PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52 and pTDH3 (aka GAP promoter)Available SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneTagsHIS10-TEV-HAExpressionYeastPromoterGAL1Available SinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastPromotersynthetic promoter containing yeast, mammalian, a…Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAHYB-GFP
Plasmid#85074PurposeExpression of GFP in Pichia pastorisDepositorInsertGFP
ExpressionYeastPromoterAOX1Available SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHZ1493
Plasmid#222102PurposeCloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pND003
Plasmid#200949PurposeYeast surface display destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast surface displayTagsAGA2, HA, and MycExpressionYeastPromoterGAL1Available SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT166_GalL_A3A-BE3
Plasmid#145116PurposeExpressing base editor A3A-BE3 in yeast cellsDepositorInsertA3A-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-hphMX6
Plasmid#19342DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SNAP-KanMX6
Plasmid#87024PurposeTag S. pombe genes in C terminal with SNAPDepositorInsertSNAP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only
