8,350 results
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Plasmid#224092PurposeYeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selectionDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2B-55
Plasmid#177924PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
ExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterTDH3Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIIIA/MS2-1
Plasmid#220631PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTB*-thymosinB-8His
Plasmid#111146PurposeExpresses human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integrationTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-URA-C4
Plasmid#160339PurposeHigh-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1093:SpRY
Plasmid#172188PurposeGenome editing of C. albicansDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIVh
Plasmid#81091PurposeSingle Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
ExpressionBacterial and YeastPromoterGAL1 GAPDHAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEmCitrine-Hygro
Plasmid#44645DepositorInsertyEmCitrine
ExpressionYeastMutationA206RAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
YEplac181-RTS1
Plasmid#8626DepositorAvailable SinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
LT1_36_QUEEN-2m
Plasmid#177708PurposePlasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)DepositorInsertpTEF1-QUEEN2m
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2679
Plasmid#241724PurposeConstruction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSD marker for selection on blasticidin SDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2681
Plasmid#241726PurposeConstruction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus sourceDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSII41N
Plasmid#194524PurposeLow copy vector backbone with nourseothricin selectivity, contains MCSDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSII42N
Plasmid#194525PurposeHigh copy vector backbone with nourseothricin selectivity, contains MCSDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS402-nicastrin/APH-1a-HA
Plasmid#207804PurposeYeast integrative vector for expression of Nicastrin and APH-1a-HADepositorInsertGamma-secretase subunits nicastrin and Aph1a
ExpressionYeastAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
720-2µ-maxHIS3
Plasmid#40612DepositorInsertHIS3 (HIS3 Synthetic, Budding Yeast)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
Plasmid#86424PurposePCR tagging module with yeast codon optimized mTurquoise2DepositorInsertmTurquoise2
ExpressionYeastAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymNeongreen-CaURA3
Plasmid#125703PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3.DepositorInsertymNeongreen
ExpressionYeastAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
ExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTG1*-thymosinB-8His
Plasmid#111147PurposeExpresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.DepositorInsertactin gamma 1 (ACTG1 Human)
UsePichia pastoris integration vectorTagsThymosin beta and a His-tagExpressionYeastMutationcodon-optimised for expression in Pichia pastorisPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWL078 MCP-2xGFP
Plasmid#197047PurposepCYC-NLS-MCP-2xGFP on pRS305DepositorInsertMCP-2xGFP
ExpressionYeastPromoterCYC1Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2PHR
Plasmid#28244PurposeExpresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activationDepositorAvailable SinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-CRY2-uTEV1Δ(220-242)
Plasmid#135452PurposeYeast expression of uTEV1Δ fused to CRY2 and mCherryDepositorInsertmCherry-CRY2-TEV1Δ(220-242)
TagsmCherryExpressionYeastPromoterGalAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
KBB520
Plasmid#185102Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFPDepositorInsertPOM152
TagsGFPExpressionYeastMutationPOM152(1-200)-GFPAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB464
Plasmid#185097PurposeHA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminusDepositorInsertPOM152
TagsGFPExpressionYeastMutationPom152DGlyc-GFPAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CuGFP-ATG8(416)
Plasmid#49423PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterCUP1Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymScarletI-CaURA3
Plasmid#118457PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.DepositorInsertymScarletI
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PTEF1-yEGFPCLN2PEST-pRS406
Plasmid#64406PurposeTEF promoter driven GFP expressionDepositorInsertTEF1 promoter (TEF1 Budding Yeast)
TagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastPromoterTEF1 promoterAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy3 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pANCH2-Nat
Plasmid#87249Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)DepositorInsertNat
ExpressionYeastAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available SinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
RPL3-GFP (96)
Plasmid#24040DepositorAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZJOM31
Plasmid#133645PurposeIntegration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVS2
Plasmid#33144DepositorInsertsLacO (256 copies)
GAL-GFP-kd200-GALpA reporter
ExpressionYeastMutationGFP is mutated such that amino acids 64-73 (FSYGV…Promoterbidirectional GAL1-GAL10 promoterAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPS22B-Cl
Plasmid#160431PurposeExpresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoterDepositorInsertRPS22B (RPS22B Budding Yeast)
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to change nucleo…PromoterGAL1 and S. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-R-GFP
Plasmid#11953DepositorInsertUb-R-GFP
ExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Arginine at…Available SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 ymBaojin-T2A-mCherry
Plasmid#219857Purposeequimolar expression of ymBaojin and mCherry in budding yeastDepositorInsertymBaojin-T2A-mCherry
ExpressionYeastAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natCX
Plasmid#73893Purposefission yeast drug selection markerDepositorInsertnatCX
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#172196PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sitesDepositorInsertp10B2-AT110
ExpressionYeastPromoter10B2 (P1 specific promoter)Available SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IQ-compete reporter (integrating)
Plasmid#247176PurposePlasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.DepositorInsertGFP-TCS-Quencher-CL1
ExpressionYeastPromoterTEF1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
DHFR-TEV (integrating)
Plasmid#247218PurposePlasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertDHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Atp5-DHFR-TEV (integrating)
Plasmid#247219PurposePlasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertAtp5 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Oxa1-DHFR-TEV (integrating)
Plasmid#247220PurposePlasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertOxa1 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWJ 1781 URA v-Src FL
Plasmid#245255PurposeExpression of full-length viral Src in yeastDepositorInsertFull-length viral Src
ExpressionYeastMutationwild-typeAvailable SinceSept. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWJ 1781 URA
Plasmid#245252PurposeProtein expression in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2B-56
Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
ExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only