8,025 results

1. pYZ174

   Plasmid

   #98421

   Purpose

   Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCT2-HRP

   Plasmid

   #44021

   Depositor

   Dane Wittrup

   Article

   Lipovsek et al Chem Biol. 2007 Oct;14(10):1176-85.

   Insert

   HRP
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

3. YIp ADE2-HIS3

   Plasmid

   #122904

   Purpose

   ADE2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTDH3-dCas9

   Plasmid

   #46920

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gRNA-ura-HYB

   Plasmid

   #64330

   Purpose

   ura3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of ura3 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAV126

   Plasmid

   #63192

   Purpose

   pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIIIA/MS2-2

   Plasmid

   #220632

   Purpose

   For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRN1120

   Plasmid

   #101750

   Purpose

   Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAR-Ec318

    Plasmid

    #130871

    Purpose

    A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas9-EcRT-GAL-Kan

    Plasmid

    #232092

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 426Gal-FUS-YFP

    Plasmid

    #29592

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG416-GAL-FUS-G156E

    Plasmid

    #181716

    Purpose

    Galactose inducible expression of FUS-G156E

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G156E

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS931

    Plasmid

    #123053

    Purpose

    TetR bacterial repressor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    TetR-NLS [3a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pILGFP5EA4

    Plasmid

    #185879

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXNubA22-Dest

    Plasmid

    #105087

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

    Type

    Empty backbone

    Use

    Tags

    NubA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPL5608_TEF1*-Cre_TRP1

    Plasmid

    #60931

    Purpose

    Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    Mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p405ADH1

    Plasmid

    #15966

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    p405ADH1 (ADH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    ADH1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Gal:mCherry-CRY2-uTEV1Δ(220-242)

    Plasmid

    #135452

    Purpose

    Yeast expression of uTEV1Δ fused to CRY2 and mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-CRY2-TEV1Δ(220-242)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Gal

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-HDEL

    Plasmid

    #129098

    Purpose

    For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

    Depositor

    Wei-Lih Lee

    Article

    Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

    Insert

    TEF1p-SS-3xGlyAla-GFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGHYB

    Plasmid

    #87447

    Purpose

    Pichia pastoris expression plasmid

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRL_CT_GI_del1

    Plasmid

    #98744

    Purpose

    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Inserts

    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    SV40-NLS, VP64-AD, and synTALE-DBD

    Expression

    Yeast

    Mutation

    mutated nucleotide 576 C to A, deleted amino acid…

    Promoter

    FBA1 and TDH3

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge