7,933 results
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Plasmid#133646PurposeIntegration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-ELO3-mCherry (ELO3 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p
Plasmid#158141PurposeExpresses Aga1p in yeastDepositorInsertAga1p
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Budding Yeast, Mouse)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1
Plasmid#101084PurposeGA biosensor expression plasmidDepositorInsertGPS1
UseTags6HIS and cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-3xHA
Plasmid#188935PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP422
Plasmid#26845DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAP004
Plasmid#160911PurposeE.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastorisDepositorInsertlacZ selection cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM151
Plasmid#133643PurposeIntegration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.DepositorInsertp1k-COX4-GFP (COX4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPOM033_P7_3"His5
Plasmid#216458Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''his5
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM026_P2_pNMT1
Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM019_P2_pRPL2102
Plasmid#216444PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rpl2102
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM015_P2_pADH1
Plasmid#216440PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter adh1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM013_P2_pENO101
Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymScarletI
Plasmid#193960PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeastDepositorInsertymScarletI
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHS3-msGFPx3
Plasmid#105257PurposePlasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.DepositorInsertChs3 portion (CHS3 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorInsertp1k-PEX1-2GFP (PEX1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC533
Plasmid#135435PurposeYeast integrating plasmid for pTEF1-mCherry reporter geneDepositorInsertmCherry
UseTagsExpressionYeastMutationcodon optimized for S. cerevisiaePromoterpTEF1Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ183
Plasmid#98425PurposePombe Expression Vector - nmt41 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ184
Plasmid#98426PurposePombe Expression Vector - nmt81 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
DdMCU
Plasmid#67642PurposeDdMCU-Flag in pACT2 vector human codon optimisedDepositorInsertDdMCU
UseTagsFlag tag with linkerExpressionYeastMutationPromoterADH1Available sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)
Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorInsertVph1-2GFP (VPH1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-nCas9(D10A)-PmCDA1
Plasmid#79617PurposeExpresses nCas9(D10A)-PmCDA1 in yeast cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB
Plasmid#14142DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSAC200
Plasmid#187911Purposepsac200-crispey-bar-vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAC212
Plasmid#187912Purposepsac212-crispey-bar-ade2KODepositorInsertade2 KO CRISPEY cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ157
Plasmid#98413PurposePombe Expression Vector - nmt81 promoter with Sp.ura4 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS10
Plasmid#107924PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES835
Plasmid#87946PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYX233-DHFR
Plasmid#163761PurposeGal-inducible yeast expression of cytosolic DHFRDepositorInsertDHFR (Dhfr Mouse)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP-ura4MX6
Plasmid#49183Purposegenomic mRFP tagging in S. pombeDepositorTypeEmpty backboneUseTagsmRFPExpressionYeastMutationPromoterAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-hpSC_URA3
Plasmid#22317DepositorInsertURA hairpin
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZJOM160
Plasmid#133670PurposeIntegration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.DepositorInsertpTPI1-mTagBFP2-SKL
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
p404-BrdU-Inc
Plasmid#71790PurposeBrdU-incorporating plasmid with selectable marker TRP1DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-H50Q
Plasmid#181724PurposeGalactose inducible expression of Alpha-synuclein-H50QDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationH50QPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9 stopGFP
Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM31
Plasmid#133645PurposeIntegration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.DepositorInsertp1k-TGL3-GFP (TGL3 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionWorm and YeastMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRR-ERalpha-5Z
Plasmid#23061DepositorInsertERalpha (ESR1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNH605-PACE-YFP
Plasmid#172002PurposeFluorescent reporter for Rpn4 activityDepositorInsert4xPACE-YFP
UseVector for genomic integration into the yeast gen…TagsExpressionYeastMutationPromoterCYC1 core promotor with 4 tandem repeats of Rpn4 …Available sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-SNF7-mCherry (SNF7 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AC
Plasmid#108686PurposePlasmid for assembly of donor DNA templates consisting of two expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGKB
Plasmid#85077Purposeexpression in Pichia pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAPAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only