8,025 results

1. pCE41

   Plasmid

   #174433

   Purpose

   C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-6xGLY-FLAG-kanMX6

   Plasmid

   #20751

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMVS102_P3-GFP-NATMX6

   Plasmid

   #99048

   Purpose

   EGFP reporter with six binding sites for the Zif268 DNA binding domain

   Depositor

   Barak Cohen

   Article

   Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

   Inserts

   McIsaac 2014 P3 promoter
   eGFP
   clonNAT resistance
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL1 promoter with 4 GAL4 sites removed and repla…

   Promoter

   McIsaac 2014 P3 promoter

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIp TRP1-HIS3

   Plasmid

   #122912

   Purpose

   TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROS17

   Plasmid

   #107931

   Purpose

   TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. YEp195-CUP1-His-Ubi

   Plasmid

   #99540

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS307

   Plasmid

   #51785

   Purpose

   an integrating (YIp) vector with a LYS2 selectable marker

   Depositor

   David Stillman

   Article

   Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG416-Gal-PA50

   Plasmid

   #84902

   Purpose

   induce expression of dipeptide repeat PA in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PA50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXP218

    Plasmid

    #26831

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT2_12_GlyRNA

    Plasmid

    #177710

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOsTIR1w/oGFP

    Plasmid

    #102883

    Purpose

    Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    OsTIR1
    

    Use

    Tags

    3x myc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNB769

    Plasmid

    #60138

    Purpose

    Red click beetle luciferase from Promega driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL FLAG103QDProGFPp303

    Plasmid

    #15587

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGJJ001

    Plasmid

    #183619

    Purpose

    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREP41-MCS+

    Plasmid

    #52690

    Purpose

    Same as pREP41, with better MCS

    Depositor

    Michael Nick Boddy

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pnmt41

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1090

    Plasmid

    #111427

    Purpose

    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS415_pGal-nCas9(D10A)

    Plasmid

    #79618

    Purpose

    Expresses nCas9(D10A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRS416 Gal Q331K YFP

    Plasmid

    #27450

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

    Plasmid

    #154817

    Purpose

    Inducible expression of AsiSI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AsiSI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCEV-G4-Km

    Plasmid

    #46819

    Purpose

    TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and HXT7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Epac-SH225

    Plasmid

    #191449

    Purpose

    expression of cAMP sensor in yeast

    Depositor

    Kees Jalink

    Insert

    mT2Del-EPACdDEPCD-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYTK-DN6

    Plasmid

    #180287

    Purpose

    HygR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - HygR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-ymNeongreen-CaURA3

    Plasmid

    #125703

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG416-Gal-PR50

    Plasmid

    #84901

    Purpose

    induce expression of dipeptide repeat PR in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    PR50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS405-FlagPEN2/PS1wt

    Plasmid

    #207803

    Purpose

    Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits PEN-2 and presenilin1 wt
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS303-PADH1-AFB2

    Plasmid

    #99530

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRR-MR-5Z

    Plasmid

    #23059

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    MR (NR3C2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N528H, Q814R, H993L

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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