7,933 results
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Plasmid#122882PurposeURA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ÂptetO7-ÂpphlO6-ÂcrtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-Kan
Plasmid#44944PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selectionDepositorInsertyomEos2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1661
Plasmid#112035PurposeOsTIR1 expression cassette marked with URADepositorInsertTIR1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS2149
Plasmid#184275PurposeTet-on genome insertion systemDepositorInsertCatrTA
UseTagsExpressionYeastMutationPromoterTDH3Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK3FS
Plasmid#85776PurposeN-ICE plasmid pK3FSDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
Plasmid#58439PurposeCassette to replace yeast promoters with an inducible promoter containing 1 lexA boxDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pND003
Plasmid#200949PurposeYeast surface display destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast surface displayTagsAGA2, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-Kan
Plasmid#44902PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selectionDepositorInsertyomKeima
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
p404-Grx5-GFP1-10
Plasmid#91952PurposeExpresses GFP1-10 in the mitochondrial matrixDepositorInsertGrx5-GFP1-10
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-T7-kanMX6
Plasmid#20760DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
UseTagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastMutationPromoterTPI1Available sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH3F-GAL1
Plasmid#85775PurposeN-ICE plasmid pH3F-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-ccdB
Plasmid#14135DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorInsertsgTEF1 promoter (TEF1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Ph
Plasmid#46816PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
LPH2782 - Ub-K48R
Plasmid#32161DepositorUseTagsExpressionYeastMutationK48RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
M4755 KanMX::LEU2 Disruptor Converter
Plasmid#51685PurposeYeast marker swap plasmid for converting KanMX3 to LEU2DepositorInsertLEU2 (LEU2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2792
Plasmid#63653PurposepCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pI4-MTU-DO-G418
Plasmid#160215PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
M3925 trp1::KanMX3 Disruptor Converter
Plasmid#51681PurposeYeast marker swap plasmid for converting Trp1 to KanMX3DepositorInsertKanMX3
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2B-57
Plasmid#177927PurposeExpresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)DepositorArticleInsertEGFP
UseTagsExpressionBacterial and YeastMutationPromoterTET3 (contains three rtTA binding sites)Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertSEC7-2GFP (SEC7 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG211
Plasmid#64519PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mGrape3
Plasmid#170265PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3DepositorArticleInsertmomeric Grape 3
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HphMx
Plasmid#122916PurposeADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9v2
Plasmid#103140PurposeTetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready siteDepositorInsertdCas9-VPR
UseTagsExpressionYeastMutationRemoved scaffold gRNA from original plasmidPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX122-mtGFP
Plasmid#45048DepositorInsertmtGFP
UseTagsExpressionYeastMutationPromoterTPIAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG
Plasmid#176396PurposeExpresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.DepositorInsertGAL1 promoter, SZ1-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-URA
Plasmid#188917PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-URA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorInsertp1k-MSP1-2GFP (MSP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYM28-BirA*
Plasmid#160290PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3DepositorInsertBirA*
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LHP398 - Ub-K48R/K63R
Plasmid#32167DepositorUseTagsExpressionYeastMutationK48R and K63RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pAG425GPD-EGFP-ccdB
Plasmid#14322DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRG216
Plasmid#64528PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ235: pRS426-His-MBP-GtFz1_Native
Plasmid#205254PurposeS. cerevisiae expression vector for GtFz1 native guideDepositorInsertGtFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-Kan
Plasmid#44943PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selectionDepositorInsertyoLSS-mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACT-Lex
Plasmid#183149PurposeYeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)DepositorInsertGAL4 AD
UseYeat two-hybridTagsHA tagExpressionYeastMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-AVT1 chr I
Plasmid#41898PurposeYeast expression of AVT1.DepositorInsertAVT1 (AVT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID3S
Plasmid#188646PurposeExpresses Target-AID3S in yeastsDepositorInsertnCas9-AID2S-UGI
UseTagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only