8,010 results

1. pJT82_GalL_nCDA1Δ198-BE3

   Plasmid

   #145046

   Purpose

   Expresses nCDA1Δ198-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ198-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-198AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64390

   Purpose

   encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416 Gal M337V YFP

   Plasmid

   #27449

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCfB4778

   Plasmid

   #106125

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-mMaple3-kanMX6

   Plasmid

   #105165

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   mMaple3-kanMX6
   

   Use

   Tags

   GlySerGly-mMaple3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL-RPB1

   Plasmid

   #10981

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Wild type RPB1 gene

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. M3929 his3::KanMX3 Disruptor Converter

   Plasmid

   #51679

   Purpose

   Yeast marker swap plasmid for converting HIS3 to KanMX3

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   KanMX3
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHES941

   Plasmid

   #87942

   Purpose

   Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCCL-ReDNAP (Y427A)

   Plasmid

   #52346

   Purpose

   CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   Recoded TP-DNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   REV1

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pI1-MTU-DO-HIS

    Plasmid

    #160142

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pI4-MTU-DO-HPH

    Plasmid

    #160216

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG413 GAL ORF1 Shuffle1 NLS

    Plasmid

    #145794

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock

    Article

    Payero et al Journal of the South Carolina Academy of

    Insert

    ORF1 Shuffle1 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Enhanced Nucleur Localization Signal

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG425 GAL Pong TPase L418A, L420A

    Plasmid

    #145795

    Purpose

    mPing Yeast Transposition Assay

    Depositor

    Charles Nathan Hancock, Susan Wessler

    Article

    Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

    Insert

    Pong TPase L418A, L420A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L418A, L420A

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. p415 Glo3

    Plasmid

    #112651

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pABY-c25

    Plasmid

    #201769

    Purpose

    Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-URA-Ub-G76A-Rnq1-GFP

    Plasmid

    #21052

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP and ubiquitin

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICXNH3

    Plasmid

    #49020

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG425GPD-ccdB-HA

    Plasmid

    #14250

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDB24

    Plasmid

    #91959

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EZ-L439

    Plasmid

    #169146

    Purpose

    Encodes OptoINVRT7 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. M3938 trp1::ADE2 Disruptor Converter

    Plasmid

    #51673

    Purpose

    Yeast marker swap plasmid for converting TRP1 to ADE2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    ADE2 (ADE2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADE2

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p426*-LbSgH

    Plasmid

    #136992

    Purpose

    Helper plasmid for LbCpf1 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHRW40

    Plasmid

    #130973

    Purpose

    HyPr plasmid with nptII marker

    Depositor

    Chris Todd Hittinger

    Article

    Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

    Insert

    empty backbone
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRY003

    Plasmid

    #81043

    Purpose

    HO endonuclease expression

    Depositor

    John McCusker

    Insert

    HO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pNXgate32-3HA

    Plasmid

    #105076

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    3xHA and NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA6a-link-yomKeima-CaURA3

    Plasmid

    #44885

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pH

    Plasmid

    #64178

    Purpose

    harbours marker gene HIS3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJOG417

    Plasmid

    #105341

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJOG418

    Plasmid

    #105342

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

    Plasmid

    #178989

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LD209

    Plasmid

    #69182

    Purpose

    Integration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg9-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB9336 (pgRNA_II-1_NatMX)

    Plasmid

    #161589

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. YIp ADE2-LEU2

    Plasmid

    #122905

    Purpose

    ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge