7,933 results

1. yHS1-M7A

   Plasmid

   #159151

   Purpose

   Cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   yHS1-M7A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of yHS1 to A…

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH243

   Plasmid

   #121147

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   humanized Streptococcus pyogenes Cas9
   gRNA expression module
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-ReDNAP (Y427A)

   Plasmid

   #52346

   Purpose

   CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

   Depositor

   Chang Liu

   Article

   Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

   Insert

   Recoded TP-DNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   REV1

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. YEp195-CUP1-His-Smt3

   Plasmid

   #99538

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZA_CeTRIC-B1_Del

   Plasmid

   #87344

   Purpose

   Inducible expression of CeTRIC-B1 in P.Pichia strains

   Depositor

   Zhenfeng Liu

   Article

   Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

   Insert

   TRIC-B1
   

   Use

   Tags

   polyhistidine

   Expression

   Yeast

   Mutation

   Δ248-297

   Promoter

   AOX1

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mito-yHS1

   Plasmid

   #159153

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAG425GPD-ccdB-HA

   Plasmid

   #14250

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5538 pUG2-SSA1

    Plasmid

    #1247

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1998 Jul 10;94(1):73-82.

    Insert

    Ssa1 (SSA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-GBP-hphMX6

    Plasmid

    #105159

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-hphMX6
    

    Use

    Tags

    GBP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHES822

    Plasmid

    #89193

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIM700

    Plasmid

    #74639

    Purpose

    alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pV1010

    Plasmid

    #111423

    Purpose

    gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NM R2E2 GFP

    Plasmid

    #1208

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    2 additional copies of second oligopeptide repeat…

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIplac204-TC-OM45-FKBPx4

    Plasmid

    #104472

    Purpose

    Integrating plasmid for overexpression of OM45-FKBPx4.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    OM45p (OM45 Budding Yeast)
    

    Use

    Tags

    FKBPx4- HA

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG304GAL-ccdB-EGFP

    Plasmid

    #14183

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG424GPD-ccdB-EYFP

    Plasmid

    #14224

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCggRO-reporter

    Plasmid

    #124582

    Purpose

    Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    CggR promoter element
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXP741

    Plasmid

    #46059

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pM

    Plasmid

    #64179

    Purpose

    harbours marker gene MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YIplac211-KAR2-msGFP2-HDEL

    Plasmid

    #160468

    Purpose

    Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    KAR2-msGFP2-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-link-yoPSmOrange-Kan

    Plasmid

    #44951

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ClhN-p1k-Vrg4-V5-APEX2-URA

    Plasmid

    #207071

    Purpose

    Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Vrg4
    

    Use

    Tags

    V5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pVRG4

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ClhN-4V5-APEX2-URA

    Plasmid

    #207056

    Purpose

    For ORF tagging with 4V5-APEX2

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    4xV5-APEX2
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ClhN-pADH3-4V5-APEX2-URA

    Plasmid

    #207063

    Purpose

    Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    4xV5-APEX2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ClhN-pADH3-Tgl3-4V5-APEX2-URA

    Plasmid

    #207066

    Purpose

    Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Tgl3
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS416 Gal TDP43 WT

    Plasmid

    #27458

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pPTK002-2-pGAP

    Plasmid

    #84961

    Purpose

    Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Glyceraldehyde-3-phosphate dehydrogenase promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1805 c to g)

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p426 25Q GPD

    Plasmid

    #1181

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pCfB4666

    Plasmid

    #106144

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p42Hyg 1-F GW

    Plasmid

    #58551

    Purpose

    Multicopy vector with Hygromycin resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMM0553

    Plasmid

    #128989

    Purpose

    LEU2 integration vector of pZF(3BS)-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    Leu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ZJOM103

    Plasmid

    #133664

    Purpose

    Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CUP Cyc8 YFP p316

    Plasmid

    #15594

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    CYC8 (CYC8 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pAV0478

    Plasmid

    #133498

    Purpose

    Expression of sfGFP targeted to the nucleus with two NLS signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPICZac-STE2-1D4

    Plasmid

    #133437

    Purpose

    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S/C252A

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LHP1463 - INP52

    Plasmid

    #32432

    Depositor

    Linda Hicke

    Article

    Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

    Insert

    INP52 (INP52 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Lac

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only