8,265 results
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Plasmid#50719Purposeexpression of human TPI1 in yeastDepositorAvailable SinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only
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LPH2782 - Ub-K48R
Plasmid#32161DepositorExpressionYeastMutationK48RPromoterCUP1Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK004
Plasmid#171276PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPgpdA ANIA_08041 promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS316CUP RNQ CFP
Plasmid#1171DepositorAvailable SinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
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p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6627
Plasmid#106159PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES822
Plasmid#89193PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
ExpressionYeastPromoterpZAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EMCV_3C
Plasmid#201941PurposeExpresses EMCV 3C protease from a GAL promoter with a URA3 markerDepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
ExpressionYeastPromotermin pCYC1Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)
Plasmid#173453PurposeContains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJH1260
Plasmid#36913DepositorInsertsScURA3
ScURA3
ExpressionBacterial, Mammalian, and Y…Available SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mCFP-kanMX6
Plasmid#105145PurposeYeast gene targetingDepositorInsertmCFP-kanMX6
TagsmCFPExpressionBacterial and YeastMutationA206KAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-TagRFP-SpHis5
Plasmid#44854PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selectionDepositorInsertyoPA-TagRFP
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HRV-B14_3C
Plasmid#203466PurposeExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 markerDepositorAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and GAL10Available SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P81nmt1-3FLAG
Plasmid#19347DepositorInsertnatMX6
ExpressionYeastAvailable SinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoClover-Kan
Plasmid#44952PurposeYeast tagging vector to create gene fusion with yeast optimized Clover with Kan selectionDepositorInsertyoClover
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB9336 (pgRNA_II-1_NatMX)
Plasmid#161589PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymVenus-CaURA3
Plasmid#118459PurposeYeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection. *An updated version of this plasmid is available: #168058 pFA6a-link-ymVenus-URA3.DepositorInsertymVenus
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKT0090
Plasmid#8714DepositorInsertyEVenus
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …Available SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046409
Plasmid#153939PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
POM42-mCherry
Plasmid#69856PurposeN-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
ExpressionYeastAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG417
Plasmid#105341PurposeYeast two hybridDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG418
Plasmid#105342PurposeYeast two hybridDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
ExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372AAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV1081
Plasmid#111425PurposeSolo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1DepositorInsertCaCas9/sgADE-NT2
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAL 25Q+ProGFPp416
Plasmid#15580DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKT0149
Plasmid#8740DepositorInsertyESapphire
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S72A, Y145F, T203IAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP622
Plasmid#26849DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046233
Plasmid#149032PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYX232-mtGFP
Plasmid#45052DepositorInsertmtGFP
ExpressionYeastPromoterTPIAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDA183
Plasmid#74247PurposepSTL1-dPSTRr(2,1)DepositorInsertpSTL1-dPSTRr(2,1)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ222
Plasmid#52223PurposeConstitutive expression of humanized Cas9DepositorInsertCas9
ExpressionYeastMutationsilent mutation in the original CspCI sitePromoteradh1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
LHP2260 - HA-INP53
Plasmid#32198DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Gal-Rad51
Plasmid#33230DepositorAvailable SinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pL2/GZ
Plasmid#1282DepositorInsertGRE-lacZ
Tagsglucocorticoid response elemen-lacZExpressionYeastAvailable SinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV1010
Plasmid#111423PurposegRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)DepositorInsertsgADE-NT2
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP255
Plasmid#78934PurposePromoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUB23-M-beta-gal
Plasmid#8816DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationM (methionine) at amino terminus of beta-galAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterTEF1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAL 46Q+ProGFPp416
Plasmid#15581DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM100
Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only