8,173 results
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Plasmid#166097PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT0090
Plasmid#8714DepositorInsertyEVenus
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …Available SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB-EGFP
Plasmid#14189DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p42Nat 1-F GW
Plasmid#58549PurposeMulticopy with clonNat resistance marker for single Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-GFP
Plasmid#166836PurposeExpresses Vac8-GFP in yeasts.DepositorAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
3322_pETcon_SARS2_Omicron-BA2
Plasmid#184409Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-EYFP-ccdB
Plasmid#14348DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
Tags6xHisExpressionYeastMutationG951A,C957AAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
416Gal-SV40NLS-FUS-YFP
Plasmid#29595DepositorAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsExpressionYeastAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-EYFP-ccdB
Plasmid#14346DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
ExpressionYeastAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
Tagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ222
Plasmid#52223PurposeConstitutive expression of humanized Cas9DepositorInsertCas9
ExpressionYeastMutationsilent mutation in the original CspCI sitePromoteradh1Available SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pUB23-M-beta-gal
Plasmid#8816DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationM (methionine) at amino terminus of beta-galAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPOP
Plasmid#59526PurposeVector for pop-in/pop-out gene replacement in Pichia pastoris.DepositorTypeEmpty backboneExpressionYeastPromoterGAPAvailable SinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
TagsGFPExpressionBacterial and YeastPromoterPRC1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-12Pk-LEU2MX6
Plasmid#49179Purposegenomic 12Pk epitope tagging in S. pombeDepositorTypeEmpty backboneTags12PkExpressionYeastAvailable SinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1
Plasmid#58436PurposeInduces Citrine expression from a promoter containing 3 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)3-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-URA3
Plasmid#122907PurposeADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
TagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSir1
Plasmid#107447PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet1
Plasmid#107444PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet1
TagsV5-HisExpressionYeastPromoterGAL1Available SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-SEC4
Plasmid#18844DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKT0150
Plasmid#8741DepositorInsertyESapphire
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S72A, Y145F, T203IAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV0749
Plasmid#133483PurposeExpression in fission yeast from promoter ptdh1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-UBQLN2
Plasmid#181708PurposeGalactose inducible expression of UBQLN2DepositorInsertUBQLN2 (UBQLN2 Human)
ExpressionYeastAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS423-TyrSgH
Plasmid#163973PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-SpHis5
Plasmid#44845PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selectionDepositorInsertyomKO2
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB-HA
Plasmid#14242DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Opi1 + terminator
Plasmid#58836PurposeExpresses GFP-Tagged Opi1 + Terminator in yeastDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-Phleomycin
Plasmid#188930PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-Phleomycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(A503V)
Plasmid#1152DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB-DsRed
Plasmid#14362DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTH728-CEN-RLuc/maxCFLuc
Plasmid#38212DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-HaloTag-DRS2
Plasmid#177103PurposeExpresses HaloTag-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
ExpressionYeastAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB305-K3L-vac
Plasmid#20053DepositorInsertK3L
ExpressionYeastAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUDE731
Plasmid#103008PurposeS. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3pDepositorInsertFncpf1
Tags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only