8,265 results
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Plasmid#133668PurposeIntegration of COX4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.DepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
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pI4-MTU-DO-URA
Plasmid#160025PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-URA
Plasmid#160020PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLS410
Plasmid#239123PurposeMinimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMXDepositorTypeEmpty backboneUseCentromericExpressionBacterial and YeastAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
Plasmid#45931DepositorInsertPCP-PS
Tags2x-yeGFPExpressionYeastPromoterMet25Available SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPIC9K_A2AR_316_V229C_R291A
Plasmid#246917PurposeExpress A2AR mutants in Pichia PastorisDepositorInsertAdenosine A2A receptor (ADORA2A Human)
Tags10xHis and alpha-factor secretion signal, FLAGExpressionYeastMutationtruncated to aa 1-316, V229C, R291APromoterAOX1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB
Plasmid#14154DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM136
Plasmid#133660PurposeIntegration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterGPDAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-mIAA7-mNeon-HISMX6
Plasmid#247607PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmNeonGreenExpressionYeastAvailable SinceDec. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterINO4Available SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
TagsmCherryExpressionYeastPromoterpBAD/tetOAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and ā¦Available SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414-ATG41
Plasmid#113080PurposeContain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.DepositorInsertATG41
ExpressionBacterial and YeastPromoterATG41 endogenous promoterAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
TagsparS segmentsExpressionYeastPromoterTEFAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaHygB
Plasmid#189564PurposeCaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicansDepositorInsertHygB
ExpressionYeastAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterHIS3Available SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
ExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, ā¦Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXN22-Dest
Plasmid#105085PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubG-3xHAExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
TagsGFPExpressionBacterial and YeastPromoterPRC1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimizā¦PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorInsertyeast enhanced GFP
ExpressionYeastPromoterpCYC1Available SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS118
Plasmid#234878PurposeCRISPR-Cas9 plasmid for use in Candida albicans. Contains Cas9, NEUT5L integration site, and the sgRNA cloning site (SNR52 promoter)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB
Plasmid#14133DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP
Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningExpressionYeastAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS1244
Plasmid#247644PurposeInducible CRISPRi-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS1005
Plasmid#247641PurposeConstitutive CRISPRa-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS1074
Plasmid#247642PurposeConstitutive CRISPRi-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS1242
Plasmid#247643PurposeInducible CRISPRa-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYES2/103Q
Plasmid#1385DepositorInserthuntingtin (103Q) (HTT Human)
TagsEGFP and FLAGExpressionYeastMutationExon1 sequences containing the first 17 amino aciā¦Available SinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA2B1
Plasmid#181714PurposeGalactose inducible expression of hnRNPA2B1DepositorInserthnRNPA2B1 (HNRNPA2B1 Human)
ExpressionYeastAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCdOpt-BMX
Plasmid#203929PurposeThis plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiaeDepositorInsertBleMX
ExpressionYeastMutationBleMX was codon optimized for Candida albicans orā¦Available SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated recoā¦TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2
Plasmid#28243PurposeExpresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activationDepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
ExpressionYeastAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUDP025
Plasmid#103874PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactisDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
UseCRISPRExpressionYeastPromoterScTDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB
Plasmid#14139DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-V5_pRS415
Plasmid#199667Purposeexpresses LOV-Turbo in the yeast cytosolDepositorInsertLOV-Turbo
TagsV5ExpressionYeastPromoterGal1,10Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_34_sfpHluorin
Plasmid#177707PurposePlasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)DepositorInsertpTEF1-sfpHluorin
UseUsed as donor in genome integration after lineariā¦ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT7
Plasmid#18847DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mIAA7-mCherry-HIS3MX6
Plasmid#247624PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmCherryExpressionYeastAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mIAA7-mNeon-hphNT1
Plasmid#247631PurposeYeast gene tagging with mIAA7DepositorInsertmIAA7
TagsmNeonGreenExpressionYeastAvailable SinceDec. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXN21-Dest
Plasmid#105086PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only