8,265 results
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Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH605-PACE-YFP
Plasmid#172002PurposeFluorescent reporter for Rpn4 activityDepositorInsert4xPACE-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of Rpn4 …Available SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mPing
Plasmid#145787PurposemPing Yeast Transposition AssayDepositorInsertmPing
ExpressionYeastMutationWTAvailable SinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB
Plasmid#14155DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNH605-CCAAT-YFP
Plasmid#172132PurposeFluorescent reporter for HAP complex activityDepositorInsert4xCCAAT-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of the H…Available SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneTagsGFP-HisExpressionYeastPromoterGAL1Available SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
p403-BrdU-Inc
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDUP3
Plasmid#92174PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.DepositorInsertNAT1
UseGenbank: u03440.1ExpressionYeastAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleAvailable SinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
Tagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLS416
Plasmid#239127PurposeMinimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorTypeEmpty backboneUseCentromericExpressionBacterial and YeastAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastPromoterGPDAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS303H-Padh-OsTIR1(F74G)
Plasmid#247625PurposeExpression of OsTIR1DepositorInsertOsTIR1
ExpressionYeastMutationF74GPromoterADH1Available SinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGBKCg
Plasmid#20162DepositorTypeEmpty backboneTagsGal4 DBDExpressionYeastAvailable SinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP2-Kan
Plasmid#44899PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selectionDepositorInsertyomTagBFP2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGADCg
Plasmid#20161DepositorTypeEmpty backboneTagsGal4 AD and SV40 NLSExpressionYeastAvailable SinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9_VP64
Plasmid#49013Purposeencodes yeast-optimized dCas9_VP64 synthetic transcription factorDepositorInsertdCas9_VP64 (codon-optimized for expression in S. cerevisiae)
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionYeastPromoterpTPGI (galactose+aTc inducible)Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGKB
Plasmid#85077Purposeexpression in Pichia pastorisDepositorTypeEmpty backboneExpressionYeastPromoterGAPAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
ExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-GAL1
Plasmid#85778PurposeN-ICE plasmid pK3FS-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR196
Plasmid#36029DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaKan
Plasmid#189565PurposeCodon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selectionDepositorInsertCaKan
ExpressionYeastAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
Plasmid#51690PurposeYeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisGDepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS418
Plasmid#11256DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGWKS7
Plasmid#139414PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.DepositorInsertmRuby3
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry
Plasmid#203656PurposeYeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsyEmCherryExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
ExpressionBacterial and YeastPromoterGAL1Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM4 (U6 in pSE360)
Plasmid#111281PurposeU6 snRNA coding sequence in pSE360(Ura) plasmidDepositorInsertSNR6
ExpressionYeastAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
ExpressionYeastAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
HSP104-b/Leu(WT)
Plasmid#1156DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-EGFP
Plasmid#14187DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_gRNA_handle_RPR1t
Plasmid#49014PurposegRNA expression empty vector (yeast)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastPromoterpRPR1Available SinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS244
Plasmid#34982DepositorAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pDM291-tetR'-tup11Δ70
Plasmid#41028DepositorInserttetR'-tup11Δ70
ExpressionYeastMutationtup11 starts at aa70Available SinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pND003
Plasmid#200949PurposeYeast surface display destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast surface displayTagsAGA2, HA, and MycExpressionYeastPromoterGAL1Available SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only