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8,173 results

Showing: 251 - 300 of 8173 results

  4. pJRoC30_Tv9nL

    Plasmid
    #188069
    Purpose
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    79 PROSS mutations, described in publication
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188069-plasmid-map-sequence-id-370129

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  5. ZJOM31

    Plasmid
    #133645
    Purpose
    Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-TGL3-GFP (TGL3 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133645-plasmid-map-sequence-id-265890

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  6. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GalL
    Available Since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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  9. pBHM2681

    Plasmid
    #241726
    Purpose
    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source
    Depositor
    Hiten Madhani
    Article
    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241726-plasmid-map-sequence-id-479856

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  10. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  11. pDB5322

    Plasmid
    #204832
    Purpose
    An integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
    Depositor
    Li-Lin Du
    Article
    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404
    Inserts
    tetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW4 promoter
    Available Since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204832-plasmid-map-sequence-id-405720

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  12. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74215-plasmid-map-sequence-id-139305

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  13. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Promoter
    Tef1
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

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  16. pAS1NB m Rosella I

    Plasmid
    #71247
    Purpose
    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.
    Depositor
    Mark Prescott
    Article
    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20
    Insert
    Rosella
    Tags
    yeast mitochondrial targeting sequence of citrate…
    Expression
    Yeast
    Promoter
    PGK1
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    71247-plasmid-map-sequence-id-363461

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  20. ZJOM90

    Plasmid
    #133647
    Purpose
    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-mCherry-HDEL
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133647-plasmid-map-sequence-id-292102

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  25. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  33. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  36. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  40. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  47. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S, C252A
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133422-plasmid-map-sequence-id-267645

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  48. pFS554

    Plasmid
    #242854
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW3 promoter
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242854-plasmid-map-sequence-id-491391

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  49. pFS553

    Plasmid
    #242853
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW2 promoter
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242853-plasmid-map-sequence-id-484488

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  50. pFS555

    Plasmid
    #242855
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW4 promoter
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242855-plasmid-map-sequence-id-484485

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Showing: 251 - 300 of 8173 results