8,265 results
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Plasmid#164917PurposeDivergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizerDepositorInsertP(PGK1)-citrine and P(HIS4)-mCherry
ExpressionYeastPromoterPGK1 (yeast) and HIS4 (yeast)Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3043(gRNA XI-1)
Plasmid#73285PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mYFP
Plasmid#105141PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mYFP
Tags41nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1
Plasmid#12388DepositorInsertgreen fluorescent protein
ExpressionYeastAvailable SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB4782
Plasmid#106141PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
TagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-NYFP
Plasmid#99553PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA1
Plasmid#46789PurposeTo express Fun30 in trans in budding yeastDepositorAvailable SinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-ymNeongreen pDRF1-GW
Plasmid#168067PurposeFRET fusion between ymScarletI and ymNeongreen for use in yeastDepositorInsertymScarletI-ymNeongreen
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KEX2-iGFPx6
Plasmid#105264PurposePlasmid for pop-in/pop-out replacement of Kex2 with Kex2-iGFP-x6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterMET17Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
ExpressionYeastPromoterRPL4AAvailable SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCplac111-yVps23-mCherry
Plasmid#45925DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0623
Plasmid#128998PurposepRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2DepositorInsertConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0624
Plasmid#128999PurposepZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConREDepositorInsertConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0621
Plasmid#128996PurposepRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1DepositorInsertConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1
ExpressionYeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB3049(gRNA XII-4)
Plasmid#73291PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCHKU38.2-2.2
Plasmid#110670PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
ExpressionYeastAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-EGFP-ccdB
Plasmid#14307DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNC891
Plasmid#20397DepositorInsertYFP-reporter
ExpressionYeastMutationX=M (see schematic)Available SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMGF173
Plasmid#96999PurposeNLS-GFP-lacZ leu1 insertion vectorDepositorInsertlacZ
TagsNLS-GFPExpressionYeastPromoterPnmt1Available SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F55
Plasmid#23079DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9R …Available SinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F31
Plasmid#23067DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed lysine 14 to Glutamine K14QAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
p2HG/N795
Plasmid#1284DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPS2045
Plasmid#1376DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
AausFP1-T2A-mCherry pdrf1-GW
Plasmid#168071PurposeProduces equimolar expression of AausFP1 and mCherry for use in yeastDepositorInsertAausFP1-T2A-mCherry
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ube2m
Plasmid#31423DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYes2-AtPCS1
Plasmid#49766PurposeExpresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces CerevisiaeDepositorAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJA053
Plasmid#128515PurposeExpresses SFA8 in PichiaDepositorInsertSFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
ExpressionYeastPromoterAOX1Available SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCitrine
Plasmid#69855PurposeN-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4929
Plasmid#126751PurposeExpresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413DepositorInsertALD6
ExpressionYeastPromoterpTRX2_5xUASAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCfB2400
Plasmid#67552PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDTN3997
Plasmid#113337PurposeMulti-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolyticaDepositorInsertmulti-copy 2-pyrone synthase
ExpressionYeastAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDP123
Plasmid#107269PurposepUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
ExpressionYeastPromoterScTDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ381
Plasmid#59899PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI siteAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVEG17*
Plasmid#111856PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)DepositorInsertGAL1p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL1pAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB-EYFP
Plasmid#14223DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-Scs2
Plasmid#58834PurposeExpresses GFP-Tagged Scs2 in yeastDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-2TAG
Plasmid#158138PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW tdTomato-T2A-mTurquoise2
Plasmid#118439PurposeProduces equimolar expression of tdTomato and mTq2DepositorInserttdTomato-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d_GgPCFT
Plasmid#165414Purposeexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.DepositorAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-EYFP-ccdB
Plasmid#14343DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only