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pENTR5'_ubi

Plasmid

#27320

Purpose

Gateway entry vector containing 3.5 kb ubi promoter fragment, with L4 and R1 sequences for MultiSite Gateway cloning

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Publication

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry in lens

Depositor

Joachim Berger, Peter Currie

Publication

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Publication

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Publication

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Publication

Di Donato et al Genome Res. 2016 May;26(5):681-92. doi:

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Expression

Zebrafish

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Purpose

MultiSite Gateway assembly vector, to generate transgenic zebrafish with ubiquitous expression of EGFP

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Promoter

–3.5 kb zebrafish ubiquitin (ubi) promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Purpose

Gateway entry vector containing ubi:loxP-EGFP-loxP. Used to assemble ubi:Switch construct.

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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zbFGF

Plasmid

#12309

Depositor

James Thomson

Publication

Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Insert

Fgf2 (fgf2 Zebrafish)

Expression

Bacterial

Mutation

Ala 11 mutated to Pro, Ser 55 mutated to Asn

Available since

Sept. 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Publication

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Mutation

Codon Optimized

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Publication

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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p5E-kdrl

Plasmid

#78687

Purpose

kdrl promoter for Gateway cloning

Depositor

Jau-Nian Chen, Sarah Childs

Publication

Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

Insert

kdrl promoter (kdrl Zebrafish)

Use

Unspecified

Promoter

kdrl

Available since

June 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Publication

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Promoter

gfap

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFRT-TODestGFP_RNAseH1

Plasmid

#65784

Purpose

expresses GFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Human, Zebrafish)

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

elavl3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependent Cas9-GFP expression

Depositor

Michael Brand

Publication

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pT7tyrgRNA

Plasmid

#46761

Depositor

Wenbiao Chen

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tyr gRNA (tyr Zebrafish)

Use

CRISPR

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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