2,162 results

1. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. zWnt5b

   Plasmid

   #21282

   Depositor

   Randall Moon

   Article

   Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

   Insert

   Wnt5b (wnt5b Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2CG2-ubi:QFGal4-SV40pA

   Plasmid

   #155119

   Purpose

   Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   ubi:QFGal4 (ubb Neurospora crassa, Zebrafish, Budding Yeast)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin B

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

   Plasmid

   #140892

   Purpose

   Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

   Depositor

   Yasushi Okamura

   Article

   Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

   Insert

   CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed 223L to F in Dr-VSP (zebrafish TPTE)

   Promoter

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-zTRAP

   Plasmid

   #42236

   Depositor

   Stephen L Johnson

   Article

   Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

   Insert

   EGFP-Rpl10a (rpl10a Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. ins:CFP-NTR

   Plasmid

   #66593

   Purpose

   Specific and conditional cell ablation of beta cells in the zebrafish pancreas

   Depositor

   Didier Stainier

   Article

   Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

   Insert

   Insulin promotor driving CFP-nitroreductase
   

   Use

   Zebrafish expression

   Tags

   CFP fused with N-terminus of nitroreductase

   Expression

   Mutation

   Promoter

   Zebrafish Insulin promoter (approximately 1.2kb)

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSB-CMV-MCS-Puro cPLA2-EGFP

   Plasmid

   #162568

   Purpose

   Used as a measure of cPLA2 activation in live cells with an EGFP tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Wnt promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK051 p5E exorh:mCerulean

   Plasmid

   #195952

   Purpose

   Gateway p5E vector with reporter

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2pACryGFP

    Plasmid

    #64022

    Purpose

    Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

    Depositor

    Joachim Berger, Peter Currie

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    GFP
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    alpha-Acrystallin promoter

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ubi:Zebrabow

    Plasmid

    #118219

    Purpose

    For ubiquitous expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p160Tol2-her4.3:switchCas9

    Plasmid

    #227680

    Purpose

    Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    loxP-dsRed-loxP-Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and her4.3

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

    Plasmid

    #230075

    Purpose

    Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT2-4xUAS:NICD-P2A-mRFP

    Plasmid

    #127594

    Purpose

    Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Insert

    NICD (notch1a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2-Opto-zFGFR1a

    Plasmid

    #232639

    Purpose

    Zebrafish FGFR1a receptor kinase domain fused to VfLOV

    Depositor

    Katherine Rogers

    Insert

    zFGFR1a kinase domain + VfLOV domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRISM-TagRFP-gcry-TagBFP

    Plasmid

    #117765

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPRISM-Stop-gcry-eGFP

    Plasmid

    #117773

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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