We narrowed to 446 results for: DHFR
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Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianPromoterCbhAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralPromotertetONAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPRT::DHFR-D
Plasmid#58528Purposereplace TgUPRT with DHFR by homologous recombinationDepositorInsertDHFR cassette
UseGateway CloningMutationpyrimethamine resistantPromoterDHFRAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX233-DHFR
Plasmid#163761PurposeGal-inducible yeast expression of cytosolic DHFRDepositorAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR(DD)-YFP
Plasmid#29325DepositorInsertDHFR(DD)-YFP
UseRetroviralTagsHA and YFPMutationChange Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …Available SinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…Available SinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2-DHFR
Plasmid#163759PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFRDepositorInsertb2-DHFR (Dhfr Budding Yeast, Mouse)
ExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR-HiBiT / Lyt2
Plasmid#179731PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sitesDepositorInserthuman DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
UseRetroviralTagsHiBiTPromoterLTR/MMLVAvailable SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-DHFR Mm
Plasmid#83073PurposeExpresses the full length DHFR Mm in E.coliDepositorAvailable SinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pERB221
Plasmid#61502Purposeconst. CenpB-GFP-Halo + mCherry-DHFRDepositorInsertsCenpB-GFP-Halo
mCherry-DHFR
TagsHaloTag and mCherryExpressionMammalianAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Budding Yeast, Mouse)
ExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRPromoterDHFR-TS 5' UTRAvailable SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-DHFR
Plasmid#233893Purposelentiviral overexpression vector for DHFRDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-ecDHFR (WT)
Plasmid#73188Purposebacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-DHFR-3xFLAG
Plasmid#233892Purposelentiviral overexpression vector for DHFRDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-DHFR-3xFLAG
Plasmid#217428PurposeLentiviral overexpression of human DHFRDepositorInsertDHFR (DHFR Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationInsert sequence is a codon-optimized gBLOCK (IDT)PromoterCMVAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRC03
Plasmid#114682PurposeDHFR-sfGFP.Strand11DepositorInsertDHFR-sfGFP.Strand11
TagsGSTPromoterT7 PromoterAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hDHFR
Plasmid#184707Purposebacterial expression of human DHFRDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgDHFR
Plasmid#233898Purposelentiviral vector expressing Cas9 and an sgRNA targeting DHFRDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DHFR
Plasmid#183281PurposeAll-in-One CRISPRko system with a guide RNA that targets DHFR geneDepositorInsertDHFR
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHFR-TEV (integrating)
Plasmid#247218PurposePlasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertDHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5-DHFR
Plasmid#78706PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker.DepositorInsertsGCaMP5
DHFR
PromoterToxoplasma SAG1 5' UTRAvailable SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
TagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryPromoterDHFR 5' UTRAvailable SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHFR
Plasmid#64200PurposeExpression of E. coli DHFRDepositorInsertDihydrofolate reductase
TagsnoneExpressionBacterialAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-NL-DHFR-SpCas9
Plasmid#124522PurposeExpresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cellsDepositorInsertNL-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-NLC-DHFR-SpCas9
Plasmid#124523PurposeExpresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cellsDepositorInsertNLC-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DHFR.cHSF1
Plasmid#134739PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable SinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationW22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM1160c2
Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1674
Plasmid#159726PurposeExpress MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPTLV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1479a3
Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHFR-C-HiBiT
Plasmid#207119PurposeBacterially expressed DHFR-HiBiT for Ni-NTA purificationDepositorInsertHuman-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
Tags10xHis-tag and HiBiT tagExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7Available SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A DHFR.cHSF1
Plasmid#134737PurposeGateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseGateway CloningTagsDHFRMutationDeletion of amino acids 186-202PromoterNoneAvailable SinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DHFR.cHSF1
Plasmid#134738PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable SinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_WTTS
Plasmid#91227Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR
Plasmid#101109PurposeEncodes dCas9(N)-DHFR fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)-DHFR
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
Tagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pERB217
Plasmid#61500PurposeConst. Halotag-GFP-Mito + mCherry-DHFRDepositorInsertsHaloTag-GFP-Mito
mCherry-DHFR
TagsHaloTag and mCherryExpressionMammalianAvailable SinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB219
Plasmid#61501PurposeConst. Halotag-GFP-PACT + mCherry-DHFRDepositorInsertsHaloTag-GFP-PACT
mCherry-DHFR
TagsHaloTag and mCherryExpressionMammalianAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
ExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Atp5-DHFR-TEV (integrating)
Plasmid#247219PurposePlasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertAtp5 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Oxa1-DHFR-TEV (integrating)
Plasmid#247220PurposePlasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertOxa1 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only