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We narrowed to 446 results for: DHFR

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Showing: 1 - 50 of 446 results

  1. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

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  6. pGEMS-II

    Plasmid
    #204765
    Purpose
    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204765-plasmid-map-sequence-id-408664

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  11. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  13. pBMN DHFR-HiBiT / Lyt2

    Plasmid
    #179731
    Purpose
    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites
    Depositor
    James Inglese
    Publication
    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23
    Insert
    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    Use
    Retroviral
    Tags
    HiBiT
    Promoter
    LTR/MMLV
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    179731-plasmid-map-sequence-id-361671

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  19. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

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  30. pLoxP-DHFR-mCherry

    Plasmid
    #70147
    Purpose
    Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
    Depositor
    David Sibley
    Publication
    Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    a selectable dihydrofolate reductase-thymidylate synthase marker
    Use
    Expression in toxoplasma gondii
    Tags
    mCherry
    Promoter
    DHFR 5' UTR
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    70147-plasmid-map-sequence-id-423191

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  40. DHFR-C-HiBiT

    Plasmid
    #207119
    Purpose
    Bacterially expressed DHFR-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human-dihydrofolate reductase-HiBiT-10xHis (DHFR Human)
    Tags
    10xHis-tag and HiBiT tag
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207119-plasmid-map-sequence-id-417165

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  45. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Publication
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E. coli dihydrofolate reductase
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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Showing: 1 - 50 of 446 results