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We narrowed to 154 results for: EcoRI

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Showing: 1 - 50 of 154 results

  1. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  6. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRI
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  7. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  9. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  20. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

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  23. pDME1T500

    Plasmid
    #220987
    Purpose
    In vitro transcription of T7A1-EcoRI-O1-t500
    Depositor
    Laura Finzi
    Insert
    T7A1-EcoRI-O1-t500
    Use
    Unspecified
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    220987-plasmid-map-sequence-id-438977

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  24. YEpADE1

    Plasmid
    #22495
    Depositor
    Beth Montelone
    Insert
    ADE1 (ADE1 Budding Yeast)
    Use
    Yeast shuttle
    Mutation
    Original 4067 bp HindIII-HindIII insertion (see Y…
    Available Since
    Jan. 20, 2010
    Availability
    Academic Institutions and Nonprofits only
    22495-plasmid-map-sequence-id-10339

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  29. pDME1rrnBT1

    Plasmid
    #211949
    Purpose
    in vitro transcription
    Depositor
    Laura Finzi
    Insert
    T7A1-EcoRI-O1-rrnBT1
    Use
    Unspecified
    Promoter
    T7A1
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    211949-plasmid-map-sequence-id-423496

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  32. pDME1λT1-trpA

    Plasmid
    #211951
    Purpose
    in vitro transcription
    Depositor
    Laura Finzi
    Insert
    T7A1-EcoRI-O1-λT1trpA
    Use
    Unspecified
    Promoter
    T7A1
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    211951-plasmid-map-sequence-id-423498

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  33. pDME1rrnBT1-T7Te

    Plasmid
    #211950
    Purpose
    in vitro transcription
    Depositor
    Laura Finzi
    Insert
    T7A1-EcoRI-O1-rrnBT1-T7Te
    Use
    Unspecified
    Promoter
    T7A1
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    211950-plasmid-map-sequence-id-423497

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  34. pscAAV-Tyr

    Plasmid
    #135616
    Purpose
    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Available Since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135616-plasmid-map-sequence-id-296005

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  35. pSG5-hAR(16)

    Plasmid
    #89120
    Purpose
    mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out
    Depositor
    Elizabeth Wilson
    Article
    Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.
    Insert
    hAR (AR Human)
    Expression
    Mammalian
    Mutation
    internal EcoRI site removed
    Promoter
    SV40
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  39. BcLOV4 fusion tools screening plasmid #2

    Plasmid
    #174512
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Article
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Insert
    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174512-plasmid-map-sequence-id-357202

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  40. mouse ptc probe M2-3

    Plasmid
    #58701
    Purpose
    makes mouse ptc probe for in situ hybridization
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    In situ probe
    Expression
    Bacterial
    Mutation
    only EcoRI fragment (5' end)
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. lucMAPT-GenRep

    Plasmid
    #214674
    Purpose
    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214674-plasmid-map-sequence-id-424937

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  42. MSCV-mMDM2-IRES-GFP

    Plasmid
    #66954
    Purpose
    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site
    Depositor
    Martine Roussel
    Insert
    Mdm2 (Mdm2 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    MSCV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66954-plasmid-map-sequence-id-173086

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  47. pG5ElbLuc

    Plasmid
    #89341
    Purpose
    The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.
    Depositor
    Roger Davis
    Article
    Seth et al J Biol Chem. 1992 Dec 5;267(34):24796-80
    Type
    Empty backbone
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    89341-plasmid-map-sequence-id-176438

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Showing: 1 - 50 of 154 results