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We narrowed to 5,097 results for: puc

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Showing: 1 - 50 of 5097 results

  2. pUC-ODN

    Plasmid
    #208642
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208642-plasmid-map-sequence-id-418350

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  3. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  4. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  10. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  13. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

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  17. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  18. pUCX

    Plasmid
    #60681
    Purpose
    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression
    Depositor
    David Ackerley
    Article
    Prosser et al Biotechnol Lett. 2014 Sep 26.
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60681-plasmid-map-sequence-id-108962

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  31. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available Since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  35. pUC19_CDC45_dTAG

    Plasmid
    #232733
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232733-plasmid-map-sequence-id-465446

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  36. pUC19_GINS4_dTAG

    Plasmid
    #232735
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232735-plasmid-map-sequence-id-465448

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  37. pUC19_MCM2_dTAG

    Plasmid
    #232731
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232731-plasmid-map-sequence-id-465447

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  38. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Promoter
    GAPDH
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    117226-plasmid-map-sequence-id-224760

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  44. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  47. pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

    Plasmid
    #163755
    Purpose
    piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes
    Depositor
    Kurt Pfannkuche
    Article
    Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1
    Insert
    mCherry
    Use
    Piccybac transposon
    Tags
    puromycin-N-acetyltransferase
    Expression
    Mammalian
    Promoter
    human NCX1
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163755-plasmid-map-sequence-id-326668

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Showing: 1 - 50 of 5097 results