Addgene

We narrowed to 9,995 results for: AGA

Showing: 451 - 500 of 9995 results

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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM sh2 DNMT1

Plasmid

#88885

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.2 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AagRNA

Plasmid

#121953

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b tracrRNA/crRNA duplex

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-3'UTR

Plasmid

#136042

Purpose

UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-CDS

Plasmid

#136043

Purpose

UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PB533_15F11-sfGFP

Plasmid

#167526

Purpose

H4K20me1-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

Insert

15F11-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 1

Plasmid

#131345

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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MLSE-shREN

Plasmid

#105583

Purpose

retrovirally express control shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDrop

Plasmid

#184872

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMTIKA

Plasmid

#22061

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

mCMV-TetOn expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

Plasmid

#194248

Purpose

Expresses 3 miRNAs targeting mouse alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(mouse) (Snca Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHTIKA

Plasmid

#22062

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

hCMV-TetOn expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pCol-TGM-luc.1309

Plasmid

#32720

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

luciferase

Use

Mouse Targeting and RNAi

Tags

Expression

Mutation

Promoter

TRE and Tre

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
double_sgRNA targeting e3 and e7

Plasmid

#190689

Purpose

sgRNAs targeting enhancer 3 and 7 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 3 and 7 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS4

Plasmid

#228450

Purpose

LS4 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS4

Use

Tags

10xHis-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His-SUMO-LS12

Plasmid

#228451

Purpose

LS12 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS12

Use

Tags

10xHis-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDIV151

Plasmid

#226180

Purpose

Expresses Cas9 in a backbone compatible with K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

Cas9

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Mutation

Promoter

GAP1

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM_HcBOP1ainsitu

Plasmid

#136529

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1a

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_ZmaBOP1binsitu

Plasmid

#136531

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_ZmaBOP2binsitu

Plasmid

#136532

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog2b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM_HcBOP1binsitu

Plasmid

#136530

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LEP-shTADA1.1226

Plasmid

#105567

Purpose

retrovirally express TADA1 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MLSE-shRPA3.457

Plasmid

#105584

Purpose

retrovirally express RPA3 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

RPA3 shRNA #457

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LEP-shTAF4.2818

Plasmid

#105563

Purpose

retrovirally express TAF4 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LEP-shTAF2.4392

Plasmid

#105562

Purpose

retrovirally express TAF2 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF2 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB404

Plasmid

#74798

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-CCNE1-HA-puro

Plasmid

#212669

Purpose

Express tagged cyclin E1

Depositor

Tobias Meyer, Yumi Konagaya

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

CCNE1 (CCNE1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB453

Plasmid

#74835

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB501

Plasmid

#74843

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB513

Plasmid

#74855

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB551

Plasmid

#74881

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB517

Plasmid

#74859

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB510

Plasmid

#74852

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB413

Plasmid

#74807

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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