4,132 results

1. pCuo CA Rac1

   Plasmid

   #84643

   Purpose

   constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively Active, Q61L

   Promoter

   cumate-inducible promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-sfCherry(1-10)

   Plasmid

   #70222

   Purpose

   Expresses sfCherry(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS2

   Plasmid

   #12177

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSSmKate2-N1

   Plasmid

   #31867

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPalmitoyl-mTurquoise2

   Plasmid

   #36209

   Purpose

   In vivo visualization of the membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   p63(1-29) (ARHGEF25 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-FOXO3a TM

   Plasmid

   #1788

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   T32A, S253A, S315A (no longer detectably phosphor…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA GSK3 beta K85A pcDNA3

    Plasmid

    #14755

    Depositor

    Jim Woodgett

    Article

    Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

    Insert

    GSK3 beta K85A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K85A. Kinase dead mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMXs-IP GFP-WIPI-1

    Plasmid

    #38272

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3xFLAG-ATF6

    Plasmid

    #11975

    Purpose

    For mammalian expression of human ATF6 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUltra-Chili-Luc

    Plasmid

    #48688

    Purpose

    3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

    Depositor

    Malcolm Moore

    Article

    Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mIFP-N1

    Plasmid

    #54620

    Purpose

    Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

    Depositor

    Xiaokun Shu, Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Type

    Empty backbone

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Use

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRBad-Z-CspGFP

    Plasmid

    #40730

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    Leucine Zipper prime - C super positive Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FR_HNF1A

    Plasmid

    #31104

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR4 no ccDB (686-1)

    Plasmid

    #17424

    Purpose

    Gateway entry vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5/Lac

    Available since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Kaede-N1

    Plasmid

    #54726

    Purpose

    Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Kaede

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag-HA-USP10

    Plasmid

    #22543

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP10 (USP10 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-hs-EGR1

    Plasmid

    #52724

    Purpose

    retroviral expression of human EGR1

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Flag-HA-OTUD5

    Plasmid

    #22610

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cdk4-HA

    Plasmid

    #1876

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-minCMV-mCherry

    Plasmid

    #27970

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge