179,521 results

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Publication

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Publication

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pLNHA-C1-HsXRN1

Plasmid

#66596

Purpose

expresses lambdaN-HA-tagged XRN1 in mammalian cells

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

XRN1 (XRN1 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-CaMPARI V398D

Plasmid

#60786

Purpose

Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Publication

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CaMPARI V398D

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

V398D

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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mPlum-N1

Plasmid

#54629

Purpose

Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Publication

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mPlum

Expression

Mammalian

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA-Wnt5A

Plasmid

#35911

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Wnt5A (WNT5A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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EF05J

Plasmid

#42941

Depositor

Eline Luning Prak

Publication

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Negative control Functionally Dead Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

ORF1 R261A, R262A

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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phage-cmv-cfp-24xpp7

Plasmid

#40652

Depositor

Robert Singer

Publication

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xPP7

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET15-MHL

Plasmid

#26092

Purpose

SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Sept. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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LeGO-iT2

Plasmid

#27343

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pcDNA Flag-RARalpha

Plasmid

#35555

Depositor

Jonathan Kurie, Harish Srinivas

Publication

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMSCVhygro-Cre

Plasmid

#34565

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pENTR4-CLIPf (w877-2)

Plasmid

#29650

Depositor

Eric Campeau

Type

Empty backbone

Use

Gateway Cloning

Tags

CLIPf

Available since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-HIF2α wt-pBabe-Puro

Plasmid

#26055

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles
pLenti6/V5-p53_R280K

Plasmid

#22933

Depositor

Bernard Futscher

Publication

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

R280K mutant

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAlbumin

Plasmid

#22037

Depositor

Didier Trono

Insert

partial sequence of human albumine

Use

Lentiviral

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Publication

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Publication

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHB9-EGFP (Hb9 promoter) [TJ#96]

Plasmid

#16275

Depositor

Thomas Jessell

Publication

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HB9 promoter

Use

Mouse Targeting

Tags

EGFP

Expression

Mammalian

Available since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLKO.1 shSCR

Plasmid

#17920

Depositor

Sheila Stewart

Publication

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles
pHA-PUMA

Plasmid

#16588

Depositor

Bert Vogelstein

Publication

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PUMA (BBC3 Human)

Tags

2x HA

Expression

Mammalian

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag Snail 6SA

Plasmid

#16221

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S96A, S100A, S107A, S111A, S115A, S119A

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLOVE-Klf4

Plasmid

#15950

Depositor

Miguel Ramalho-Santos

Publication

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

Kruppel-like factor 4 (Klf4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Publication

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Publication

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Publication

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Publication

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Publication

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Human, Synthetic, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Publication

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEYFP-N1-Pink1

Plasmid

#101874

Purpose

Transient expression of Pink1-YFP in mammalian cells

Depositor

Richard Youle

Publication

Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN induced putative kinase 1 (PINK1 Human)

Tags

fluorescent tag EYFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Publication

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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179,521 results