We narrowed to 15,037 results for: pET

1. pET-Octo_VirD2_NLS-Cas9-6xHis

   Plasmid

   #232296

   Purpose

   Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

   Depositor

   Hee-Sik Kim

   Article

   Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   Octo_VirD2_NLS-Cas9-6xHis
   

   Use

   CRISPR

   Tags

   6xHis and Octopine-type VirD2-derived NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-Caspase-3(D3A,V266E)

   Plasmid

   #90091

   Purpose

   Expresses caspase D3A and V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A V266E

   Promoter

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SUMO-AsCas12f-2NLS

   Plasmid

   #171613

   Purpose

   NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   NLS-AsCas12f1-NLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-his6-HA3-SNAPf

   Plasmid

   #188941

   Purpose

   T7 expression vector for making his6- and HA3-tagged SNAP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-SNAPf
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM11-6xHis-TEV-hORF1p_K3AK4A

   Plasmid

   #202585

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

   Promoter

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23b-Casp2-Delta Card

   Plasmid

   #11810

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 Delta Card (CASP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Delta Card

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+) 6xHis-TEV-IFIT1

   Plasmid

   #53557

   Purpose

   bacterial expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. VV020: WT Arch-3 - eGFP in pET28b

    Plasmid

    #58488

    Purpose

    Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3
    

    Use

    Tags

    eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SENP6 (catalytic domain)

    Plasmid

    #16359

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP6 catalytic domain (SENP6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 628 to 1112 catalytic domain of human …

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pET-45b-HP1a-mCherry

    Plasmid

    #185012

    Purpose

    Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    CBX5 (HP1a) (Cbx5 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS0663 pET45b(+) KraIscB-1 TwinStrep

    Plasmid

    #176536

    Purpose

    Bacterial expression of KraIscB-1-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    KraIscB-1
    

    Use

    Tags

    TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-SENP8 (full length))

    Plasmid

    #16361

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP8 full length (SENP8 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    human SENP8 full length, see Depositor Comments

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Use

    Tags

    6xHis-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET27b-ecDHFR-N23PP/L28F

    Plasmid

    #118584

    Purpose

    Bacterial expression of E.coli DHFR N23PP and L28F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L28 mutated to F28, N23 mutated to P23, a proline…

    Promoter

    T7

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Promoter

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-His6-Ubc9-K14R

    Plasmid

    #185759

    Purpose

    Bacterial expression of His6-Ubc9-K14R

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    K14R

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Synthetic, Human)
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PET28a-cpLumiLuc-Rapamycin sensor

    Plasmid

    #186292

    Purpose

    Expression Rapamycin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-Rapamycin sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TRE-YPet-WPRE

    Plasmid

    #193342

    Purpose

    AAV-mediated expression of YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28-6His-SUMO-Nsp14

    Plasmid

    #154956

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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