We narrowed to 16,885 results for: Por

1. pFREE_Amp

   Plasmid

   #92051

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast

   Plasmid

   #205557

   Purpose

   Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-PCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-Gsdmd-NT 4A

   Plasmid

   #80952

   Purpose

   Expresses Gsdmd-NT 4A mutant in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT 4A (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Use

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dClover2-C1

   Plasmid

   #54577

   Purpose

   Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

   Depositor

   Michael Davidson

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   dClover2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3.1-hRARα

   Plasmid

   #135397

   Purpose

   Human Retinoic Acid Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoic acid receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TadCBE-Cas9NG

   Plasmid

   #225093

   Purpose

   Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

   Insert

   TadCBE-Cas9NG
   

   Use

   CRISPR

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Orai1 CFP

   Plasmid

   #19757

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-22b(+) pCopA RBS30 mscarlet

   Plasmid

   #226370

   Purpose

   Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

   Depositor

   Urartu Seker

   Article

   Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

   Insert

   mScarlet
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Promoter

   copA promoter (pCopA)

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-kcnk18

    Plasmid

    #209000

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk18 (kcnk18 Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E228Q Vps4A-HA

    Plasmid

    #200087

    Purpose

    3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    E228Q

    Promoter

    CMV

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Clover-Geminin(1-110)

    Plasmid

    #83915

    Purpose

    Fluorescent probe for M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115268

    Purpose

    Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    EiCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CX-nestinGFPas

    Plasmid

    #32401

    Depositor

    Patrick Salmon, Henryk Zulewski

    Article

    Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

    Insert

    nestin promoter/enhancer GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. phMYT1L-N106

    Plasmid

    #31782

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    myelin transcription factor 1-like (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p.UTA.3.0 Empty

    Plasmid

    #82447

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

    Plasmid

    #176310

    Purpose

    Expresses CreN59-nMag-NLS with mCherry marker.

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1alpha

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZa pBAD csgA CBDcex pLac Ara csgGEF

    Plasmid

    #226378

    Purpose

    Plasmid carries csgA and csgG,E,F genes of curli operon

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    csgA and csgG,E,F genes of curli operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DsRed-rab9 WT

    Plasmid

    #12677

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSC234

    Plasmid

    #216357

    Purpose

    xhNup93-Nb3t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup93-Nb3t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-hRXRα

    Plasmid

    #135400

    Purpose

    Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using CMV promoter

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Human retinoid X receptor- alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LOV-Turbo-His6_pET21a

    Plasmid

    #199668

    Purpose

    expresses LOV-Turbo in bacteria

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. P427-FBP_13

    Plasmid

    #139704

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-CaMKII-GRAB_g5-HT3.0

    Plasmid

    #208723

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBN1

    Plasmid

    #86710

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

    Insert

    Plmn-1::mCherry::his-58::pie-1 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-3×FLAG-SLC35A2

    Plasmid

    #186283

    Purpose

    transient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

    Insert

    SLC35A2 (SLC35A2 Human)
    

    Use

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Opto-ACTU−

    Plasmid

    #198869

    Purpose

    Optogenetic recruitment construct to increase inner membrane surface charge

    Depositor

    Peter Devreotes

    Article

    Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

    Insert

    Rad17 polyanionic tail (-14)
    

    Use

    Synthetic Biology

    Tags

    CRY2PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSyn-GRAB_g5-HT3.0mut

    Plasmid

    #208718

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTetOne-tdTomato-cNLS

    Plasmid

    #138529

    Purpose

    Inducible expression of tdTomato with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Use

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLG327

    Plasmid

    #128797

    Purpose

    Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl(s)

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAC-94N

    Plasmid

    #53281

    Purpose

    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPIDNB2

    Plasmid

    #169902

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p2H12Matry

    Plasmid

    #221143

    Purpose

    cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMRXIP Turquoise2-Syntaxin17

    Plasmid

    #89938

    Purpose

    Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    human Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTD-NStrepHis

    Plasmid

    #45936

    Purpose

    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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45. pC0045-EF1a-PguCas13b-NES-HIV

    Plasmid

    #103861

    Purpose

    Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PguCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pG108-K - AhpC-Glow

    Plasmid

    #185135

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Inserts

    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    

    Use

    Pg106

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. mKO2-C1

    Plasmid

    #54494

    Purpose

    Localization: C1 Cloning Vector, Excitation: 551, Emission: 565

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid

    #45574

    Purpose

    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    co I-Sce I
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAC-PHYTipi

    Plasmid

    #53283

    Purpose

    Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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