19,186 results

1. mTagBFP2-TOMM20-N-10

   Plasmid

   #55328

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AAV-CMV-GFP

   Plasmid

   #67634

   Purpose

   AAV vector expressing GFP

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV flag ERR alpha

   Plasmid

   #10975

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

   Insert

   ERR alpha (ESRRA Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLC-EGFP-RIP3

   Plasmid

   #75165

   Purpose

   LentiCRISPR-EGFP with sgRNA targeting human RIP3

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   RIP3 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-puro-Bcl2

   Plasmid

   #46971

   Purpose

   lentiviral expression of Bcl2

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMALB-ATF4.12

   Plasmid

   #155033

   Purpose

   Fluorescent reporter for ATF4 translation, Negative Control

   Depositor

   Peter van Galen, John Dick

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   uORF1 start codon mutated

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-MCS1-P2A-MCS2 (Hygro)

   Plasmid

   #80922

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMALB-ATF4.14

   Plasmid

   #155034

   Purpose

   Fluorescent reporter for ATF4 translation, Positive Control

   Depositor

   Peter van Galen, John Dick

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   uORF1 and uORF2 start codons mutated

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH_blast_MCS_Nard_GFP_LAMIN

    Plasmid

    #167340

    Purpose

    Expresses GFP fused to lamin A (LA) in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-rNFM

    Plasmid

    #32907

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.

    Insert

    EGFP-rat NFM fusion (Nefm Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. JD553

    Plasmid

    #160392

    Purpose

    Wheat GRF4-GIF1 chimera in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    wheat GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MDM2p-Mdm2-YFP

    Plasmid

    #53962

    Purpose

    Expresses YFP tagged Mdm2 from MDM2 promoter

    Depositor

    Uri Alon, Galit Lahav

    Article

    Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    MDM2

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIPZ-bioGEF-UBnc-PURO

    Plasmid

    #208044

    Purpose

    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    UBnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCF806-reci

    Plasmid

    #186711

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nb-gp41-GFP (MoonTag-Nb-GFP)

    Plasmid

    #128602

    Purpose

    Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and sfGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-DHX36iso1

    Plasmid

    #159585

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HOP-flash

    Plasmid

    #83467

    Purpose

    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLJC5-3XMyc-EGFP-PEX26

    Plasmid

    #139059

    Purpose

    Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XMyc-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    UBC

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-Ef1a-sRGECO

    Plasmid

    #137125

    Purpose

    Viral expression of sRGECO

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    sRGECO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Pet28a_Synthetic_Human_H2A.1

    Plasmid

    #42634

    Depositor

    Joe Landry

    Insert

    Human H2A.1 (H2AC13 Synthetic, Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Codon Optimized

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pCR4 hNFATc1-EE

    Plasmid

    #17857

    Depositor

    Jerry Crabtree

    Article

    Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2attPC

    Plasmid

    #51547

    Purpose

    DICE donor plasmid without neural transcription factors.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid

    #26285

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge