We narrowed to 8,601 results for: Ott
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Plasmid#107701Purposein vivo voltage imagingDepositorInsertQuasAr3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-zsGreen
Plasmid#110729PurposeMammalian Expression of p14ARF IRES zsGreenDepositorInsertp14ARF IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX
Plasmid#169727PurposeMammalian expression construct of GFP tagged cDAXXDepositorInsertcDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-740PromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
hCXCR4-mTFP1
Plasmid#110195PurposeEncodes for human CXCR4 coding sequence tagged with the mTFP1 FPDepositorInserthCXCR4 (CXCR4 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (396-492)
Plasmid#86755PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 396-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (181-413)
Plasmid#86754PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 181-413PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (201-492)
Plasmid#86751PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 201-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2281
Plasmid#111902PurposessAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle346 (NEFM Human)
UseAAVTagsExpressionMutationPromoterPle346 (NEFM MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-HA
Plasmid#97072PurposeExpresses CARMA2sh/CARD14 isoform 3 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsHAExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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eGFP Depdc5 pLJM1
Plasmid#46380DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
YA1629: pAAV_CAG-FLEX-paQuasAr3-s
Plasmid#107703Purposein vivo voltage imagingDepositorInsertpaQuasAr3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2159
Plasmid#70120PurposePromoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.DepositorInsertssAAV-MCS-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceMay 12, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1985
Plasmid#49116PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1986
Plasmid#49117PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)mGAT1YFP3
Plasmid#41667DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsYFPExpressionMammalianMutation“Monomerizing” YFP A206K mutation; 3 C-terminal-m…PromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Suz12-F1-GFP
Plasmid#91881PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorInsertgRNA targeting Suz12 (Suz12 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-FLAG
Plasmid#97071PurposeExpresses CARMA2sh/CARD14 isoform 3 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NOPLight-ctr
Plasmid#195579PurposeExpresses the control sensor NOPLight-ctr in mammalian cellsDepositorInsertNOPLight-ctr
UseTagsExpressionMammalianMutationD110A, D130APromoterCMVAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT 5N5A
Plasmid#154285PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationN322A, N356A, N390A, N424A, N458A mutations (HCF-…PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS423-mitoT
Plasmid#174525PurposeExpresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranesDepositorInsertYeast mitochondrial intermembrane tether
UseSynthetic BiologyTagseGFPExpressionYeastMutationPromoterMET25Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA
Plasmid#47456PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.DepositorInsertTALE(Grm2)-GS-CIB1(NLS*,∆318-334)
UseAAV; TaleTagsHAExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shDsup
Plasmid#90024PurposeTo establish Dsup knock down cellsDepositorInsertDsup
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSynapsin1_NOPLight1
Plasmid#195580PurposeExpresses NOPLight1 in neuronsDepositorInsertNOPLight1
UseAAVTagsExpressionMammalianMutationPromoterhuman Synapsin-1Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-H_N1
Plasmid#85058PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarlet-HExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold
Plasmid#121817PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag Depdc5 pLJM1
Plasmid#46336DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP4
Plasmid#129507PurposeExpresses AvicFP4 constitutively in E. coli (most strains)DepositorInsertAvicFP4
UseTagsExpressionBacterialMutationOne of two variants of AvicFP4 tested with essent…Promotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MKV937/nlp-1p::FlincG3
Plasmid#140508PurposeThis construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.DepositorInsertFlincG3
UseTagsExpressionWormMutationPromoternlp-1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Vimentin-7
Plasmid#57688PurposeLocalization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVimentin (VIM Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5 Flag Grb10 5A short
Plasmid#37484DepositorInsertGrb10 5A (GRB10 Human)
UseTagsFlagExpressionMammalianMutationisoform C (missing N-term 58aa), 536aa, S150A T15…PromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase Non-Cleavable
Plasmid#59826PurposeAllows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationNon-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter
Plasmid#121814PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 VAMP2 R125TAG
Plasmid#69876Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2-GFPDepositorInsertVAMP2-GFP (Vamp2 Rat)
UseSynthetic BiologyTagsGFPExpressionMammalianMutationAmber stop codon in the linker between VAMP2 and …PromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-SERCA1
Plasmid#109498PurposeMuscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertSERCA1 (atp2a1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold
Plasmid#121841PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expressionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag Grb10 9A long
Plasmid#37485DepositorInsertGrb10 9A (GRB10 Human)
UseTagsFlagExpressionMammalianMutationisoform A, 594aa, S104A S150A T155A S158A S426A S…PromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralTagsExpressionMutationPromoterH1, U6, 7SK, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS2112
Plasmid#49137PurposeAAV plasmid with NR2E1 (Ple264) promoter driving expression of EmGFP.DepositorInsertssAAV-Ple264-emGFP
UseAAVTagsExpressionMammalianMutationPromoterNR2E1Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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H11-mScarletALG8
Plasmid#172440PurposeHipp11 targeting vector, expresses mScarlet-mALG8DepositorInsertmScarlet-mALG8
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE
Plasmid#67290PurposeInduce human iN conversion from fibroblastDepositorInsertAsc1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS2114
Plasmid#49139PurposeAAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.DepositorInsertssAAV-Ple67-emGFP
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold
Plasmid#121816PurposepMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2cl-FLAG
Plasmid#97077PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cellsDepositorInsertCARD14 isoform 2 (CARD14 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Left TALE
Plasmid#196496PurposeExpresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Right TALE
Plasmid#196497PurposeExpresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS1990
Plasmid#49121PurposeAAV plasmid with FEV (Ple67) promoter driving expression of iCre.DepositorInsertssAAV-Ple67-iCre
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits